Nicotianamine, a novel enhancer of rice iron bioavailability to humans
Autor: | Xin-Hang Jiang, Ye Zheng, Luqing Zheng, Dennis D. Miller, Xin Gen Lei, Xiaoshu Bei, Raymond P. Glahn, Chunxiang Ai, Z. Cheng, Huixia Shou, Ross M. Welch |
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Jazyk: | angličtina |
Rok vydání: | 2010 |
Předmět: |
Crops
Agricultural Iron Science Population Biofortification Biological Availability Ferrous Nicotianamine synthase Plant Biology/Plant Genetics and Gene Expression chemistry.chemical_compound Botany medicine Humans Transgenes Food science Nicotianamine Biotechnology/Plant Biotechnology Promoter Regions Genetic education education.field_of_study Alkyl and Aryl Transferases Multidisciplinary biology food and beverages Oryza Iron deficiency Plants Genetically Modified Ascorbic acid medicine.disease Ferritin chemistry biology.protein Plant Biology/Agricultural Biotechnology Medicine Azetidinecarboxylic Acid Research Article |
Zdroj: | PLoS ONE, Vol 5, Iss 4, p e10190 (2010) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | BackgroundPolished rice is a staple food for over 50% of the world's population, but contains little bioavailable iron (Fe) to meet human needs. Thus, biofortifying the rice grain with novel promoters or enhancers of Fe utilization would be one of the most effective strategies to prevent the high prevalence of Fe deficiency and iron deficiency anemia in the developing world.Methodology/principal findingsWe transformed an elite rice line cultivated in Southern China with the rice nicotianamine synthase gene (OsNAS1) fused to a rice glutelin promoter. Endosperm overexpression of OsNAS1 resulted in a significant increase in nicotianamine (NA) concentrations in both unpolished and polished grain. Bioavailability of Fe from the high NA grain, as measured by ferritin synthesis in an in vitro Caco-2 cell model that simulates the human digestive system, was twice as much as that of the control line. When added at 1:1 molar ratio to ferrous Fe in the cell system, NA was twice as effective when compared to ascorbic acid (one of the most potent known enhancers of Fe bioavailability) in promoting more ferritin synthesis.ConclusionsOur data demonstrated that NA is a novel and effective promoter of iron utilization. Biofortifying polished rice with this compound has great potential in combating global human iron deficiency in people dependent on rice for their sustenance. |
Databáze: | OpenAIRE |
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