Sensitized enantioselective laser-induced phosphorescence detection in chiral capillary electrophoresis
| Autor: | Ivonne Lammers, Cees Gooijer, Joost B. Buijs, Freek Ariese |
|---|---|
| Přispěvatelé: | BioAnalytical Chemistry, AIMMS, LaserLaB - Analytical Chemistry and Spectroscopy |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Detection limit
chemistry.chemical_classification Chromatography Cyclodextrins Cyclodextrin Chemistry Dexter electron transfer Lasers Enantioselective synthesis Analytical chemistry Electrophoresis Capillary Stereoisomerism SDG 10 - Reduced Inequalities Photochemistry Acceptor Camphor Analytical Chemistry Resins Synthetic Capillary electrophoresis Luminescent Measurements Enantiomer Phosphorescence |
| Zdroj: | Analytical Chemistry, 82(22), 9410-9417. American Chemical Society Lammers, I, Buijs, J B, Ariese, F & Gooijer, C 2010, ' Sensitized enantioselective laser-induced phosphorescence detection in chiral capillary electrophoresis ', Analytical Chemistry, vol. 82, no. 22, pp. 9410-9417 . https://doi.org/10.1021/ac101764z |
| ISSN: | 0003-2700 |
| Popis: | The sensitivity of enantioselective cyclodextrin-induced room-temperature phosphorescence detection of camphorquinone (CQ) is enhanced using sensitization via a donor with a high extinction coefficient. The enantiomeric distinction is based on the different phosphorescence lifetimes of (+)-CQ and (-)-CQ after their complexation with α-cyclodextrin (α-CD). The collisional Dexter energy transfer from the selected donor 2,6-naphthalenedisulfonic acid (2,6-NS) to the acceptor CQ is still very efficient despite the inclusion of the acceptor into CD. For coupling to the chiral separation of (±)-CQ in cyclodextrin-based electrokinetic chromatography, the donor was added to the deoxygenated background electrolyte that consisted of 20 mM α-CD, 10 mM carboxymethyl-β-CD, and 25 mM borate buffer at pH 9.0. Time-resolved batch studies on sensitized phosphorescence show a significant enantioselectivity for (+)- and (-)-CQ in the presence of both α-CD and CM-β-CD although the lifetime difference is somewhat reduced with respect to direct excitation. The enantiomers were distinguished after their separation using an online time-resolved detection system. Excitation was performed at 266 nm with a pulsed, small-sized, quadrupled Nd:YAG laser. With 1 × 10(-5) M 2,6-NS, limits of detection of 4.1 × 10(-8) M and 5.2 × 10(-8) M were found for (+)-CQ and (-)-CQ, respectively. The online measured lifetimes were 238 ± 8 μs for (+)-CQ and 126 ± 10 μs for (-)-CQ. The method was used to determine the concentration of (±)-CQ leaching from a cured dental resin into water. The extracts contained 4.7 ± 0.1 × 10(-7) M of both (+)-CQ and (-)-CQ. |
| Databáze: | OpenAIRE |
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