Comparative analysis of DNA extraction protocols for ancient soft tissue museum samples
| Autor: | Wen Xiao, Albert Min-Shan Ko, E. Andrew Bennett, Ming Zhang, Hong Wu, Xiaotian Feng, Yichen Liu, Tilo Nadler, Qiaomei Fu, Yongqiang Wang, Li Yu, Hongru Wang, Christian Roos, Xiaowei Mao, Peng Cao, Qingyan Dai |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
High-throughput sequencing
Ecology integumentary system Ancient DNA Historical and ancient skin materials Dna concentration Soft tissue Extinct species Biology DNA extraction DNA sequencing humanities chemistry.chemical_compound QL1-991 chemistry Evolutionary biology DNA extraction method Natural degradation Animal Science and Zoology Zoology Letter to the Editor Ecology Evolution Behavior and Systematics DNA |
| Zdroj: | Zoological Research Zoological Research, Vol 42, Iss 3, Pp 280-286 (2021) |
| ISSN: | 2095-8137 |
| Popis: | DNA studies of endangered or extinct species often rely on ancient or degraded remains. The majority of ancient DNA (aDNA) extraction protocols focus on skeletal elements, with skin and hair samples rarely explored. Similar to that found in bones and teeth, DNA extracted from historical or ancient skin and fur samples is also extremely fragmented with low endogenous content due to natural degradation processes. Thus, the development of effective DNA extraction methods is required for these materials. Here, we compared the performance of two DNA extraction protocols (commercial and custom laboratory aDNA methods) on hair and skin samples from decades-old museum specimens to Iron Age archaeological material. We found that apart from the impact sample-specific taphonomic and handling history has on the quantity and quality of DNA preservation, skin yielded more endogenous DNA than hair of the samples and protocols tested. While both methods recovered DNA from ancient soft tissue, the laboratory method performed better overall in terms of DNA yield and quality, which was primarily due to the poorer performance of the commercial binding buffer in recovering aDNA. |
| Databáze: | OpenAIRE |
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