Electroanalysis of benzalkonium chloride in ophthalmic formulation by boron-doped diamond electrode
| Autor: | Jeremy D. Glennon, Alyah Buzid, Majidah Alsaeedi, John H. T. Luong, Huda Alghamdi |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Boron doped diamond
Chemistry 010401 analytical chemistry C12 homologs 02 engineering and technology Benzalkonium chloride 021001 nanoscience & nanotechnology 01 natural sciences High-performance liquid chromatography 0104 chemical sciences Analytical Chemistry Electrode Eye drops Electrochemistry medicine Electrochemical method Boron-doped diamond electrode lipids (amino acids peptides and proteins) 0210 nano-technology medicine.drug Nuclear chemistry |
| Popis: | Benzalkonium chloride (BAK) is a mixture of alkyl benzyl dimethyl ammonium chlorides, which is used primarily as a biocide, surfactant, preservative, and antimicrobial agent in the pharmaceutical industry, in particular in ophthalmologic and nasal solutions. However, BAK may cause harmful consequences on the eye structures of the anterior segment. Control of BAK identity and content is necessary by applying a sensitive detection method. This study unravels the use of a glassy carbon (GC) electrode and a pristine boron-doped diamond electrode (BDD) for the detection of four BAK homologs in a non-aqueous medium using square wave voltammetry (SWV). The BDD provided more reproducibility of the oxidation potential than GC with a correlation coefficient of 0.999. The irreversible oxidation peak was very broad and deconvoluted into 3 peaks corresponding to C12, C14, and combined C16–C18 to reflect their concentration ratio in the mixture. The method was then extended to the detection of the C12 homolog in the ophthalmic formulations with a limit of detection (LOD) of 0.4 μg/mL. The estimated BAK levels in three ophthalmic formulations were in agreement with the specified values by the manufacturers. The results were validated by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection, confirming the presence of a single homolog (C12) in the eye drops. |
| Databáze: | OpenAIRE |
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