Effect of Geranylgeranylacetone on Ultraviolet Radiation Type B-Induced Cataract in Heat-Shock Transcription Factor 1 Heterozygous Mouse
| Autor: | Yui Inomata, Yu Tezuka, Atushi Sanbe, Takashi Okuno, Kouhei Hashizume, Daijiro Kurosaka, Satoshi Ogasawara, Toshiyasu Imaizumi |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Heterozygote medicine.medical_specialty Ultraviolet Rays Blotting Western DNA Mutational Analysis Mice Transgenic Real-Time Polymerase Chain Reaction Cataract Mice 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Heat Shock Transcription Factors Cataracts Internal medicine Heat shock protein Lens Crystalline medicine Animals Inducer HSF1 Transcription factor integumentary system Chemistry fungi Dose-Response Relationship Radiation medicine.disease Sensory Systems Surgery Mice Inbred C57BL Heat shock factor Radiation Injuries Experimental Ophthalmology 030104 developmental biology Real-time polymerase chain reaction Endocrinology medicine.anatomical_structure Gene Expression Regulation Lens (anatomy) 030221 ophthalmology & optometry RNA Female Diterpenes Transcription Factors |
| Zdroj: | Current Eye Research. 42:732-737 |
| ISSN: | 1460-2202 0271-3683 |
| Popis: | Purpose: We investigated whether heat-shock transcription factor 1 (HSF1) was involved in ultraviolet radiation type B (UVR-B)-induced lens opacity (cataract) using HSF1 heterozygous mice. We also examined the effects of geranylgeranylacetone (GGA), an inducer of heat-shock proteins via activation of HSF, on the UVR-B-induced cataract.Material and Methods: Male HSF1+/– and WT mice were unilaterally exposed to UVR-B (total: 1200mJ) at 16 weeks of age. At 48 h after the last UVR-B irradiation, the lens was isolated and the induction of the cataract was quantified as the cataract area ratio (opacity area/anterior capsule). GGA was orally administered at a dosage of 500 mg/kg once a day for two days before the first UVR-B exposure until the end of the experiment (21days in total).Results: The HSF1 expression was more greatly decreased in the lens from HSF1+/– mice than in that from WT mice (p < 0.01). UVR-B exposure could mainly induce cataracts in the anterior capsule in both HSF1+/– and WT mice, whi... |
| Databáze: | OpenAIRE |
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