High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks

Autor: Guy Roger Ndong Atome, Sylvain Godreuil, Arsène Mabika Mabika, Jean Jules Tewa, Jean Constant Obague Mbeang, Paul Yannick Bitome Essono, Jean Ulrich Muandze Nzambe, Pierre Philippe Mbehang Nguema, François Bretagnolle, Richard Onanga
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Cefotaxime
Klebsiella pneumoniae
Parks
Recreational
Social Sciences
Wildlife
Pathology and Laboratory Medicine
Klebsiella Pneumoniae
chemistry.chemical_compound
Feces
Klebsiella
Medicine and Health Sciences
Prevalence
Psychology
Mammals
Multidisciplinary
biology
Animal Behavior
Enterobacteriaceae Infections
Eukaryota
Citrobacter freundii
Bacterial Pathogens
Anti-Bacterial Agents
Phenotype
Medical Microbiology
Animal Sociality
Vertebrates
Apes
Medicine
Pathogens
Mandrillus
MacConkey agar
Klebsiella Oxytoca
medicine.drug
Research Article
Primates
Gorillas
Science
Animals
Wild
Microbial Sensitivity Tests
Proteus Mirabilis
beta-Lactams
Klebsiella variicola
Microbiology
beta-Lactam Resistance
beta-Lactamases
Enterobacteriaceae
Microbial Control
Enterobacter cloacae
medicine
Escherichia coli
Animals
Gabon
Microbial Pathogens
Pharmacology
Behavior
Gorilla gorilla
Bacteria
Organisms
Biology and Life Sciences
Klebsiella oxytoca
biology.organism_classification
Proteus mirabilis
chemistry
Antibiotic Resistance
Amniotes
bacteria
Antimicrobial Resistance
Zoology
Zdroj: PLoS ONE, Vol 16, Iss 10, p e0257994 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Data on the prevalence of antibiotic resistance in Enterobacteriaceae in African wildlife are still relatively limited. The aim of this study was to estimate the prevalence of phenotypic intrinsic and acquired antimicrobial resistance of enterobacteria from several species of terrestrial wild mammals in national parks of Gabon. Colony culture and isolation were done using MacConkey agar. Isolates were identified using the VITEK 2 and MALDI-TOF methods. Antibiotic susceptibility was analysed and interpreted according to the European Committee on Antimicrobial Susceptibility Testing guidelines. The preliminary test for ESBL-producing Enterobacteriaceae was performed by replicating enterobacterial colonies on MacConkey agar supplemented with 2 mg/L cefotaxime (MCA+CTX). Extended-spectrum beta-lactamase (ESBL) production was confirmed with the double-disc synergy test (DDST). The inhibition zone diameters were read with SirScan. Among the 130 bacterial colonies isolated from 125 fecal samples, 90 enterobacterial isolates were identified. Escherichia coli (61%) was the most prevalent, followed by Enterobacter cloacae (8%), Proteus mirabilis (8%), Klebsiella variicola (7%), Klebsiella aerogenes (7%), Klebsiella oxytoca (4%), Citrobacter freundii (3%), Klebsiella pneumoniae (1%) and Serratia marcescens (1%). Acquired resistance was carried by E. coli (11% of all E. coli isolates) and E. cloacae (3% of all E. cloacae) isolates, while intrinsic resistance was detected in all the other resistant isolates (n = 31); K. variicola, K. oxytoca, K. pneumoniae, E. cloacae, K. aerogenes, S. marcescens and P. mirabilis). Our data show that most strains isolated in protected areas in Gabon are wild type isolates and carry intrinsic resistance rather than acquired resistance.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje