Analysis of Nicotine Metabolites in Hair and Nails Using QuEChERS Method Followed by Liquid Chromatography–Tandem Mass Spectrometry
Autor: | Sangwon Cha, Hyun-Deok Cho, Junhee Kim, Hosub Im, Yu Wang, Eunyoung Lee, Joon Hyuk Suh, Ji-Youn Lee, Sang Beom Han, Chang Hwa Jin |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Nicotine
Bioanalysis QuEChERS Metabolite bioanalysis Pharmaceutical Science Urine 010402 general chemistry Mass spectrometry Quechers 01 natural sciences Analytical Chemistry lcsh:QD241-441 chemistry.chemical_compound LC–MS/MS lcsh:Organic chemistry Limit of Detection Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Drug Discovery medicine Humans nail Physical and Theoretical Chemistry Cotinine Chromatography Communication Solid Phase Extraction 010401 analytical chemistry Organic Chemistry hair nicotine metabolites 0104 chemical sciences Nails chemistry Chemistry (miscellaneous) Molecular Medicine Chromatography Liquid medicine.drug |
Zdroj: | Molecules, Vol 25, Iss 1763, p 1763 (2020) Molecules |
ISSN: | 1420-3049 |
Popis: | Many studies have analyzed nicotine metabolites in blood and urine to determine the toxicity caused by smoking, and assess exposure to cigarettes. Recently, hair and nails have been used as alternative samples for the evaluation of smoking, as not only do they reflect long-term exposure but they are also stable and easy to collect. Liquid-liquid or solid-phase extraction has mainly been used to detect nicotine metabolites in biological samples; however, these have disadvantages, such as the use of toxic organic solvents and complex pretreatments. In this study, a modified QuEChERS method was proposed for the first time to prepare samples for the detection of nicotine metabolite cotinine (COT) and trans-3′-hydroxycotinine (3-HCOT) in hair and nails. High-performance liquid chromatography–tandem mass spectrometry (LC–MS/MS) was used to analyze traces of nicotine metabolites. The established method was validated for selectivity, linearity, lower limit of quantitation, accuracy, precision and recovery. In comparison with conventional liquid-liquid extraction (LLE), the proposed method was more robust, and resulted in higher recoveries with favorable analytical sensitivity. Using this method, clinical samples from 26 Korean infants were successfully analyzed. This method is expected to be applicable in the routine analysis of nicotine metabolites for environmental and biological exposure monitoring. |
Databáze: | OpenAIRE |
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