In vitro evidence of tissue susceptibility rhythms. I. Temporal variation in effect of potassium chloride and phenylephrine on rat aorta
| Autor: | Hakan Zengil, Görgün Cz, Nurettin Abacioglu, Ugur Hodoglugil, Z. S. Ercan, Zuhal Aktuna Keskil |
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| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Activity Cycles
Male medicine.medical_specialty Vascular smooth muscle Physiology Potassium chemistry.chemical_element Aorta Thoracic Potassium Chloride Phenylephrine Physiology (medical) medicine.artery Internal medicine medicine Prazosin Thoracic aorta Animals Vasoconstrictor Agents Rats Wistar Aorta business.industry Antagonist Rats Endocrinology chemistry medicine.symptom business Vasoconstriction medicine.drug Muscle Contraction |
| Popis: | In this study, time-dependent variations in the in vitro sensitivity of rat thoracic aorta rings to potassium chloride (KCl) and phenylephrine (Phe) were investigated. Animals were synchronized with a 12h light and 12h darkness.(lights on 08:00-20:00) schedule, and thoracic aortas were obtained at six different times of the day (1, 5, 9, 13, 17, and 21 hours after lights on). In order to avoid endothelial influence, all experiments were performed in endothelium-denuded preparations. Responses to KCI showed time-dependent variations in all the concentrations used. Phe-induced contractions also exhibited time-dependent differences. The rhythmic pattern of Phe responses did not change with the presence of the alpha(1)-adrenergic antagonist prazosin. In addition, both the EC50 values of KCl and Phe, and also the K-B values of prazosin, displayed rhythmicity. In conclusion, time of obtaining tissues is an important factor for experimental standardization in, at least, vascular smooth muscle preparations. |
| Databáze: | OpenAIRE |
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