ZNF667 attenuates leukocyte-endothelial adhesion via downregulation of P-selectin in skin flap following remote limb ischemic preconditioning
| Autor: | Jizhe Qiu, Yuanbin Li, Zhangfu Zhou, Zhuang Chen, Haifen Liu, Yi Tang, Taotao Cui |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male P-selectin Pharmacology Cell Line Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Edema Cell Adhesion Medicine Animals Humans Cell adhesion Ischemic Preconditioning Microvessel Oncogene Proteins Leukocyte aggregation business.industry Endothelial Cells Cell Biology General Medicine Adhesion Rats P-Selectin 030104 developmental biology 030220 oncology & carcinogenesis Reperfusion Injury Ischemic preconditioning medicine.symptom business Carrier Proteins |
| Zdroj: | Cell biology internationalREFERENCES. 45(7) |
| ISSN: | 1095-8355 |
| Popis: | We assessed the effects and potential mechanism of romote ischemic preconditioning (RIPC) on leukocytes-endothelium cell adhesion in the flap microvessel after ischemia-reperfusion (I/R) injury. Eight hours after reperfusion, edema and intravascular leukocyte aggregation were reduced and microvessels were more obvious in the group with superficial inferior epigastric artery (SIEA) perforator flap (SIEA-flap) subjected to RIPC than in the I/R group. Zinc finger protein 667 (ZNF667) was significantly increased but P-selectin was decreased in the flaps subjected to RIPC, compared to those in the I/R group. The low expression of P-selectin was associated with ZNF667 expression and activation in human dermal microvascular endothelial cells (HDMECs) in response to hypoxic preconditioning (HPC). ZNF667 bound to the P-selectin promoter region, suppressing its transcription through a special core sequence. The ablation of P-selectin by siRNA effectively prevented the leukocytes-endothelium cell adhesion effect of ZNF667-knockdown. ZNF667 upregulation attenuates leukocyte-endothelial cell adhesion by negatively regulating the expression of P-selectin in SIEA-flap subjected to RIPC. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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