Molecular identification of airborne bacteria associated with aerial spraying of bovine slurry waste employing 16S rRNA gene PCR and gene sequencing techniques
| Autor: | David A. McDowell, Juluri R. Rao, Mayumi Murayama, Paul J. Rooney, Colin E. Goldsmith, John E. Moore, Jiru Xu, Peter J.A. Moore, Y. Kakinuma, Motoo Matsuda, M. Ann S. McMahon, Yasunori Maeda, B. Cherie Millar, Anne Loughrey |
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| Rok vydání: | 2009 |
| Předmět: |
Health
Toxicology and Mutagenesis Microbacterium Air Microbiology Lysobacter Biology Polymerase Chain Reaction Waste Disposal Fluid Microbiology RNA Ribosomal 16S Animals Psychrobacter Air Pollutants Bacteria Brevundimonas Public Health Environmental and Occupational Health General Medicine Sequence Analysis DNA Ribosomal RNA 16S ribosomal RNA biology.organism_classification Exiguobacterium Pollution Manure RNA Bacterial Cattle Waste disposal Environmental Monitoring |
| Zdroj: | Ecotoxicology and environmental safety. 73(3) |
| ISSN: | 1090-2414 |
| Popis: | Polymerase chain reaction amplification of the universal 16S ribosomal RNA (rRNA) gene was performed on a collection of 38 bacterial isolates, originating from air sampled immediately adjacent to the agricultural spreading of bovine slurry. A total of 16 bacterial genera were identified including both Gram-positive and Gram-negative genera. Gram-positive organisms accounted for 34/38 (89.5%) of total bacterial numbers consisting of 12 genera and included Staphylococcus (most common genus isolated), Arthrobacter (2nd most common genus isolated), Brachybacterium, Exiguobacterium, Lactococcus, Microbacterium and Sporosarcina (next most common genera isolated) and finally, Bacillus, Brevibacterium, Frigoribacterium, Mycoplana and Pseudoclavibacter. Gram-negative organisms accounted for only 4/38 (10.5%) bacterial isolates and included the following genera, Brevundimonas, Lysobacter, Psychrobacter and Rhizobium. No gastrointestinal pathogens were detected. Although this study demonstrated a high diversity of the microorganisms present, only a few have been shown to be opportunistically pathogenic to humans and none of these organisms described have been described previously as having an inhalational route of infection and therefore we do not believe that the species of organisms identified pose a significant health and safety threat for immunocompetant individuals. |
| Databáze: | OpenAIRE |
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