Anti-apoptotic signaling and failure of apoptosis in the ischemic rat hippocampus
| Autor: | Flemming Fryd Johansen, Hans Lassmann, Georg Johannes Müller |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Male
Morphology Programmed cell death Necrosis Bioenergetic failure Apoptosis Biology Hippocampus Respiratory chain complex subunits Brain Ischemia lcsh:RC321-571 chemistry.chemical_compound Ischemia medicine Animals Colchicine HSP70 Heat-Shock Proteins Rats Wistar Cyclic AMP Response Element-Binding Protein lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Injections Intraventricular Adenosine Triphosphatases Neurons Electron Transport Complex I Superoxide Dismutase Respiratory chain complex Transcription Factor RelA Membrane Proteins Mitochondrial Proton-Translocating ATPases Granule cell Neuronal Apoptosis-Inhibitory Protein Tubulin Modulators Rats Hsp70 Cell biology Anti-apoptosis medicine.anatomical_structure Neurology chemistry nervous system Dentate Gyrus NAIP medicine.symptom Carrier Proteins Signal Transduction |
| Zdroj: | Neurobiology of Disease, Vol 25, Iss 3, Pp 582-593 (2007) |
| Popis: | Several anti-apoptotic proteins are induced in CA1 neurons after transient forebrain ischemia (TFI), but fail to protect the majority of these cells from demise. Correlating cell death morphologies (apoptosis-like and necrosis-like death) with immunohistochemistry (IHC), we investigated whether anti-apoptosis contributes to survival, compromises apoptosis effector functions and/or delays death in CA1 neurons 1-7 days after TFI. As surrogate markers for bioenergetic failure, the IHC of respiratory chain complex (RCC) subunits was investigated. Dentate granule cell (DGC) apoptosis following colchicine injection severed as a reference for classical apoptosis. Heat shock protein 70 (Hsp70), neuronal apoptosis inhibitory protein (NAIP) and manganese superoxide dismutase (MnSOD) were upregulated in the majority of intact CA1 neurons paralleling the occurrence of CA1 neuronal death (days 3-7) as well as in a proportion of apoptosis-( |
| Databáze: | OpenAIRE |
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