First-time Isolation of Flavonoids and Cytotoxic Potential of the Amazonian Shrub Ptychopetalum olacoides Benth
| Autor: | Arthur Ladeira Macedo, Rosângela de A. Epifanio, Thatyana R. A. Vasconcelos, Raquel Carvalho Montenegro, Alessandra L. Valverde, Paula C. Jimenez, Karen D. B. Dutra, Rosane N. Castro |
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| Přispěvatelé: | Universidade Federal de São Paulo (UNIFESP) |
| Rok vydání: | 2017 |
| Předmět: |
Flavonoids
P. olacoides biology Traditional medicine ved/biology Chemistry ved/biology.organism_classification_rank.species Antitumor General Chemistry biology.organism_classification Shrub In vitro medicine.anatomical_structure Ptychopetalum olacoides Cell culture medicine Cytotoxic T cell Olacaceae Fibroblast Human cancer Cancer |
| Zdroj: | Repositório Institucional da UNIFESP Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| ISSN: | 1984-6835 2402-0141 |
| DOI: | 10.21577/1984-6835.20170137 |
| Popis: | FAPERJ Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) In the present study, three flavonoids, 3-O-methylquercetin (1), 3,4'-O-dimethylquercetin (2) and 3,7-O-dimethylquercetin (3) were isolated and characterized for the first time from a methanol extract obtained from the species Ptychopetalum olacoides. The structures of compounds were identified by spectroscopic methods (1D-, 2D-NMR, MS and UV) and confirmed by comparison with the respective literature data. The cytotoxic effect of crude extract was evaluated in vitro against three human cancer cell lines. The results showed a mild cytotoxic activity (IC50 = 45.16 mu g/mL) against breast cancer (MCF-7). However, crude extract did not exhibit any cytotoxic effect against normal cell human fibroblast (MRC-5). Univ Fed Fluminense, Inst Quim, Dept Quim Organ, Campus Valonguinho, BR-24020141 Niteroi, RJ, Brazil Univ Fed Para, Inst Ciencias Biol, BR-66075110 Belem, Para, Brazil Univ Fed Sao Paulo, Dept Ciencias Mar, BR-11070100 Santos, SP, Brazil Univ Fed Rural Rio de Janeiro, Dept Quim, BR-23897000 Seropedica, RJ, Brazil Univ Fed Sao Paulo, Dept Ciencias Mar, BR-11070100 Santos, SP, Brazil Web of Science |
| Databáze: | OpenAIRE |
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