Characterization of Cancer-Induced Nociception in a Murine Model of Breast Carcinoma
Autor: | Rafael Noal Moresco, Gabriela Trevisan, Flávia Karine Rigo, Gabriele Cheiran Pereira, Sara Marchesan Oliveira, Caren Tatiane de David Antoniazzi, Carolina S Stein, Alessandra Marcone Milioli, Diéssica Padilha Dalenogare, Samira Dal-Toé De Prá, Amanda Spring de Almeida, Camila dos Santos Ritter, Diulle Spat Peres |
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Rok vydání: | 2019 |
Předmět: |
Nociception
0301 basic medicine Morpholines Analgesic Bone Neoplasms Mammary Neoplasms Animal Naphthalenes Pharmacology 03 medical and health sciences Cellular and Molecular Neuroscience Naproxen 0302 clinical medicine Breast cancer Cell Line Tumor medicine Animals Clonogenic assay Acetaminophen Pain Measurement Analgesics Mice Inbred BALB C Morphine Cannabinoids Codeine business.industry Bone metastasis Cell Biology General Medicine medicine.disease Benzoxazines Disease Models Animal 030104 developmental biology Allodynia Hyperalgesia Calcium Female medicine.symptom business Locomotion 030217 neurology & neurosurgery medicine.drug |
Zdroj: | Cellular and Molecular Neurobiology. 39:605-617 |
ISSN: | 1573-6830 0272-4340 |
Popis: | Severe and poorly treated pain often accompanies breast cancer. Thus, novel mechanisms involved in breast cancer-induced pain should be investigated. Then, it is necessary to characterize animal models that are reliable with the symptoms and progression of the disease as observed in humans. Explaining cancer-induced nociception in a murine model of breast carcinoma was the aim of this study. 4T1 (104) lineage cells were inoculated in the right fourth mammary fat pad of female BALB/c mice; after this, mechanical and cold allodynia, or mouse grimace scale (MGS) were observed for 30 days. To determine the presence of bone metastasis, we performed the metastatic clonogenic test and measure calcium serum levels. At 20 days after tumor induction, the antinociceptive effect of analgesics used to relieve pain in cancer patients (acetaminophen, naproxen, codeine or morphine) or a cannabinoid agonist (WIN 55,212-2) was tested. Mice inoculated with 4T1 cells developed mechanical and cold allodynia and increased MGS. Bone metastasis was confirmed using the clonogenic assay, and hypercalcemia was observed 20 days after cells inoculation. All analgesic drugs reduced the mechanical and cold allodynia, while the MGS was decreased only by the administration of naproxen, codeine, or morphine. Also, WIN 55,212-2 improved all nociceptive measures. This pain model could be a reliable form to observe the mechanisms of breast cancer-induced pain or to observe the efficacy of novel analgesic compounds. |
Databáze: | OpenAIRE |
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