Local resistance to oxidative stress by overexpression of copper-zinc superoxide dismutase limits neointimal formation after angioplasty
| Autor: | Michael D. Dake, Eser Yuksel, Paras Ghafouri, Paul R. Hilfiker, Isaac J. Bright, David S. Wang, Douglas N. Miniati, Michael D. Kuo |
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| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Inflammation medicine.disease_cause Sensitivity and Specificity Muscle Smooth Vascular Andrology Superoxide dismutase Lipid peroxidation chemistry.chemical_compound Random Allocation Restenosis Reference Values Angioplasty medicine Animals Radiology Nuclear Medicine and imaging Probability Neointimal hyperplasia biology business.industry Superoxide Dismutase Biopsy Needle medicine.disease Immunohistochemistry Surgery Femoral Artery Disease Models Animal Oxidative Stress chemistry Reperfusion Injury biology.protein Tissue and Organ Harvesting Female Vascular Resistance medicine.symptom Cardiology and Cardiovascular Medicine business Tunica Intima Elastin Oxidative stress Angioplasty Balloon Biomarkers |
| Zdroj: | Journal of endovascular therapy : an official journal of the International Society of Endovascular Specialists. 11(6) |
| ISSN: | 1526-6028 |
| Popis: | PURPOSE To examine the effects of oxidative stress on neointimal hyperplasia through local overexpression of human copper-zinc superoxide dismutase (Cu-Zn SOD). METHODS The left common femoral arteries (CFA) of 18 New Zealand white rabbits were subjected to balloon overdilation injury. Each dilated CFA was then incubated with either a nonviral (buffer) or viral (adenovirus overexpressing beta-galactosidase) control or an adenovirus overexpressing Cu-Zn SOD. Animals were then sacrificed at 3, 7, or 28 days (3 arteries per group per time point) and the treated CFA segments were harvested for analysis of esterase-positive inflammatory cells and extracellular matrix elements. The intima-to-media ratio (I/M) was measured to assess the degree of neointimal formation. RESULTS At 3 days, local SOD levels in the Cu-Zn SOD-treated group were significantly elevated relative to both controls (p |
| Databáze: | OpenAIRE |
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