Splenic Marginal-zone Lymphoma: One or More Entities? A Histologic, Immunohistochemical, and Molecular Study of 42 Cases
| Autor: | Vassiliki Douka, Athanasios Fassas, Dimitra Anagnostou, Chrysoula Belessi, Nikolaos Laoutaris, Theodora Papadaki, Kostas Stamatopoulos, Evi Pouliou, Achilles Anagnostopoulos, Aikaterini Parasi |
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| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Pathology Lymphoma medicine.medical_treatment DNA Mutational Analysis Splenectomy Gene Rearrangement B-Lymphocyte Heavy Chain Biology Immunophenotyping Pathology and Forensic Medicine Biomarkers Tumor medicine Gene Rearrangement B-Lymphocyte Light Chain Humans RNA Neoplasm Splenic marginal zone lymphoma Splenic Neoplasms Anatomical pathology Histology DNA Neoplasm medicine.disease Staining Immunohistochemistry Immunoglobulin Light Chains Surgery Anatomy Immunoglobulin Heavy Chains IGHV@ Spleen |
| Zdroj: | American Journal of Surgical Pathology. 31:438-446 |
| ISSN: | 0147-5185 |
| DOI: | 10.1097/01.pas.0000213419.08009.b0 |
| Popis: | We analyzed 42 splenic marginal-zone lymphoma (SMZL) cases diagnosed on splenectomy specimens after established World Health Organization criteria. A predominantly nodular growth pattern was observed in 24 cases; the remainder showed predominantly (11/42) or exclusively (7/42) diffuse infiltration. Twenty-one cases showed the "classic" biphasic appearance; 13 cases exhibited marginal-zone morphology; finally, 8 cases were composed predominantly of small cells. CD21 and CD35 were expressed by 12/42 and 17/38 cases, respectively. DBA.44 was detected in 24/42 cases. Seventeen of 37 cases were surface IgD (SIgD)-positive. Twenty-one of 22 analyzed cases were SIgM-positive (12/21 coexpressed SIgD). Five of 37 cases were SIgG-positive. CD27 staining was observed in 21/35 cases; 7/18 CD27-positive cases coexpressed SIgD; 7/14 CD27-negative cases were SIgD-positive. Forty IGHV-D-J rearrangements were amplified in 34/42 cases: the IGHV4-34 gene predominated, followed by IGHV1-2. Using the 98% homology cut-off, 25/40 (62.5%) IGHV sequences were considered as "mutated": 10/11 cases with monomorphous, marginal-zone morphology were IGHV-mutated; in contrast, 4/6 cases with monomorphous, small-cell morphology were IGHV-unmutated. Five of 7 cases expressing IGHV1 subgroup genes had biphasic morphology, whereas 6/9 IGHV3-expressing cases had monomorphous, marginal-zone morphology. Most IGHV-mutated cases (14/20; 70%) were SIgD-negative; in contrast, 8/11 IGHV-unmutated cases expressed SIgD. CD27 was detected in 10/17 IGHV-mutated and 6/10 IGHV-unmutated cases. Seven of 11 CD27-negative cases were IGHV-mutated; 5/7 CD27-negative/IGHV-mutated cases expressed DBA.44. These results confirm the considerable histologic, immunohistochemical, and molecular heterogeneity of SMZL and indicate an origin from the diverse resident B-cell populations of the normal SMZ. |
| Databáze: | OpenAIRE |
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