Detection of the oyster parasite Bonamia ostreae by fluorescent in situ hybridization
| Autor: | Daniel L. Distel, Ryan B. Carnegie, Bruce J. Barber |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Oyster
Hemocytes biology Oligonucleotide Haplosporida Hybridization probe Haplosporidium nelsoni fungi In situ hybridization Aquatic Science biology.organism_classification Ostreidae Molecular biology Host-Parasite Interactions Fishery Bonamia ostreae biology.animal Animals Parasite hosting Ostrea edulis DNA Probes In Situ Hybridization Fluorescence Ecology Evolution Behavior and Systematics |
| Zdroj: | Diseases of Aquatic Organisms. 55:247-252 |
| ISSN: | 1616-1580 0177-5103 |
| DOI: | 10.3354/dao055247 |
| Popis: | Bonamia ostreae is an economically significant protistan parasite of the flat oyster Ostrea edulis in Europe and North America. Management of this parasite depends partly upon its reliable identification in wild and aquacultured oyster populations, but B. ostreae is small and difficult to detect by traditional microscopic methods. We designed a fluorescent in situ hybridization (FISH) assay to sensitively detect B. ostreae in standard histopathological sections of B. ostreae-infected oysters using fluorescently labeled DNA oligonucleotide probes. Hybridization using a cocktail of 3 presumptively B. ostreae-specific, fluorescein iso(thio)cyanate (FITC)-labeled oligonucleotides produced an unambiguous staining pattern of small green rings inside infected oyster hemocytes that was easily distinguished from host tissue background. This pattern is diagnostic for B. ostreae. A negative control cocktail of oligonucleotides containing 2 mismatches relative to target sequences, on the other hand, failed to hybridize at all. B. ostreae-specific probes did not cross-react with a related protist, Haplosporidium nelsoni. |
| Databáze: | OpenAIRE |
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