Prolactin Binding Sites on Human Chorion-Decidua Tissue
| Autor: | Charles A. Singhas, Alan D. Rogol, Linda A. Mcwey |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
medicine.medical_specialty
endocrine system Time Factors Receptors Prolactin Magnesium Chloride Receptors Cell Surface Endogeny Peptide hormone Biology Iodine Radioisotopes Pregnancy Internal medicine Mole Decidua Humans Medicine Magnesium Receptor business.industry Cell Membrane Obstetrics and Gynecology Decidualization Chorion General Medicine Prolactin Cell biology Endocrinology medicine.anatomical_structure Membrane protein embryonic structures Osmoregulation Female business |
| Zdroj: | Obstetrical & Gynecological Survey. 38:218-219 |
| ISSN: | 0029-7828 |
| Popis: | An effective procedure has been developed and utilized to demonstrate the presence of prolactin receptors on the plasma membranes of human chorion-decidua cells. Particulate fractions from human chorion-decidua sedimenting between 1,500 and 45,000 x g display optimal binding of 215I-labeled ovine prolactin when incubated at a membrane protein concentration of 200 micrograms per assay tube for 2 hours at 22 degrees C. Specific binding was increased by pretreatment of the membrane particles with 5M magnesium chloride to remove endogenous prolactin. These receptors show binding parameters (affinity, 0.92 x 10(9) L/mode; capacity, approximately 80 fmoles/mg) similar to those of lactogenic receptors in the rabbit mammary gland and, the rabbit and rat liver. The presence of prolactin receptors in human chorion-decidua suggests that may play a role in mediating local action(s) of prolactin such as involvement in the decidualization reaction or in maintaining fetal osmoregulation. |
| Databáze: | OpenAIRE |
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