Association between the nucleolus and the coiled body
| Autor: | Robert L. Ochs, Luis Eduardo Coelho Andrade, Donna L. Gruol, Carol L. Peebles, Edward K. L. Chan, Ivan Raška, Rufus W Burlingame, Eng M. Tan |
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| Rok vydání: | 1990 |
| Předmět: |
Chromosomal Proteins
Non-Histone Nucleolus Immunocytochemistry Biology Hippocampus Cell Line Structural Biology Cerebellum Nucleolus Organizer Region Animals Humans Histone locus body Cells Cultured Organelles Fibrillarin Nuclear Proteins Anatomy Rats Cell biology Microscopy Electron Cell culture Dactinomycin Cytochemistry Coilin Nucleolus organizer region Cell Nucleolus Dichlororibofuranosylbenzimidazole HeLa Cells |
| Zdroj: | ResearcherID |
| ISSN: | 1047-8477 |
| Popis: | By means of light and electron microscopic immunocytochemistry, we have localized p80-coilin, a specific protein marker for coiled bodies, in mammalian cell lines as well as in primary rat neuron cultures. p80-coilin-stained nuclear bodies, which also contained fibrillarin, could be subsequently silver stained by a method specific for the visualization of nucleolar organizer regions. In cycling cells, most coiled bodies were not associated with nucleoli, whereas in rat neurons such an association was frequent. The treatment of cycling cells with actinomycin D or 5,6-dichloro-1-β- d -ribo furanosyl-benzimidazole led to nucleolar segregation and/or disintegration, and to an association of p80-coilin staining structures with nucleoli. p80-coilin-positive structures contained fibrillarin in both untreated and treated cells. These results support the opinion that there might be a special association between coiled bodies and nucleoli, particularly in neuronal cells. |
| Databáze: | OpenAIRE |
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