New serum biomarkers for detection of esophageal carcinoma using Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
| Autor: | Chunqin Pan, Jianmin Shen, Chibo Liu, Renyong Guo |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Spectrometry Mass Electrospray Ionization Cancer Research Pathology medicine.medical_specialty Esophageal Neoplasms Matrix assisted laser desorption ionization time of flight Mass spectrometry Peptide Mapping Magnetics Serum biomarkers Biomarkers Tumor Carcinoma medicine Humans Early Detection of Cancer Neoplasm Staging Chromatography Chemistry Esophageal disease Reproducibility of Results Blood Proteins General Medicine medicine.disease Neoplasm Proteins Surface-enhanced laser desorption/ionization Biomarker (cell) Matrix-assisted laser desorption/ionization Oncology Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Female |
| Zdroj: | Journal of Cancer Research and Clinical Oncology. 137:513-519 |
| ISSN: | 1432-1335 0171-5216 |
| DOI: | 10.1007/s00432-010-0899-3 |
| Popis: | To screen for the potential protein biomarkers in serum for the diagnosis of esophageal carcinoma (EC) using proteomic fingerprint technology.Proteomic fingerprint technology combining magnetic beads with MALDI-TOF-MS was used to profile and compare the serum proteins from 78 patients with EC and 95 healthy blood donors. Proteomic patterns associated with EC were identified by Biomarker Patterns Software. Model of biomarkers was constructed and evaluated using the Biomarker Patterns Software.A total of 60 discriminating m/z peaks were identified that were related to EC (P0.01). The model of biomarkers constructed by the Biomarker Patterns Software based on the four biomarkers (2049.6, 3936.5, 5339.9, and 13748.8 Da) generated excellent separation between the EC and control groups. The sensitivity was 92.5% and the specificity was 88%. Blind test data indicated a sensitivity of 89.5% and a specificity of 84.4%.Biomarkers for EC can be discovered in serum by MALDI-TOF-MS combining the use of magnetic beads. The pattern of combined markers provides a powerful and reliable diagnostic method for EC with a high sensitivity and specificity. |
| Databáze: | OpenAIRE |
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