Postentry Events Are Responsible for Restriction of Productive Varicella-Zoster Virus Infection in Chinese Hamster Ovary Cells
| Autor: | Guang-Yun Cai, Scott A. Simpson, Kara R. Mizokami, Bruce W. Banfield, Myron J. Levin, Lewis I. Pizer, Renée L. Finnen |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Herpesvirus 3
Human Genes Viral viruses Immunology Gene Expression CHO Cells Biology medicine.disease_cause Virus Replication Microbiology Virus Herpesviridae Receptor IGF Type 2 Cell Line Mice Species Specificity Viral entry Virology Cricetinae medicine Animals Humans Reporter gene integumentary system Chinese hamster ovary cell Varicella zoster virus virus diseases biochemical phenomena metabolism and nutrition Hydrogen-Ion Concentration Endocytosis Virus-Cell Interactions Herpes simplex virus Viral replication Insect Science NIH 3T3 Cells Receptors Virus Heparitin Sulfate |
| Popis: | Productive infection of varicella-zoster virus (VZV) in vitro is restricted almost exclusively to cells derived from humans and other primates. We demonstrate that the restriction of productive VZV infection in CHO-K1 cells occurs downstream of virus entry. Entry of VZV into CHO-K1 cells was characterized by utilizing an ICP4/β-galactosidase reporter gene that has been used previously to study herpes simplex virus type 1 entry. Entry of VZV into CHO-K1 cells involved cell surface interactions with heparan sulfate glycosaminoglycans and a cation-independent mannose-6-phosphate receptor. Lysosomotropic agents inhibited the entry of VZV into CHO-K1 cells, consistent with a low-pH-dependent endocytic mechanism of entry. Infection of CHO-K1 cells by VZV resulted in the production of both immediate early and late gene products, indicating that a block to progeny virus production occurs after the initiation of virus gene expression. |
| Databáze: | OpenAIRE |
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