Biophysical, Structural and Compositional characterization at the molecular level of Native Pulmonary Surfactant Membranes directly isolated from mice Wild-type and Knocked-out Protein D Bronco-alveolar Lavage Fluid
| Autor: | Luis A. Bagatolli, Søren Hansen, Hans Kristian Hannibal-Bach, Adam Cohen Simonsen, Jorge Bernardino de la Serna, Jens Knudsen |
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| Zdroj: | University of Southern Denmark Bernardino de la Serna, J, Hansen, S, Hannibal-Bach, H K, Simonsen, A C, Knudsen, J & Bagatolli, L 2009, ' Biophysical, Structural and Compositional characterization at the molecular level of Native Pulmonary Surfactant Membranes directly isolated from mice Wild-type and Knocked-out Protein D Bronco-alveolar Lavage Fluid ', Biophysical Journal, no. 3, pp. 451a . |
| Popis: | Pulmonary surfactant is a surface active material composed of lipids and proteins produced by type II pneumocytes cells in the alveoli. This tension-active material forms a unique interface separating gas and liquids at the alveolar cell surface, reduce surface tension close to 0mN/m and maintains lung volumes and alveolar homeostasis at the end of the expiration. Abnormalities of surfactant in the immature lung or in the acutely inflamed mature lung are related to several illnesses. There are four pulmonary surfactant proteins (SP-A, -B, -C and -D). SP-A and -D have a very important role in the immunological response against pathogens. The particular lipid composition of the lung surfactant suggests that native surfactant mono- and bi-layer-based structures could exhibit lateral segregation phenomena at physiological temperatures. The principles underlying the interfacial film and membrane-base organization are not well defined. Therefore, a deep study in the correlation among surfactant composition, structure and biophysical function is needed. The present work tries to get advantage of the combination of different biochemical and biophysical techniques applied to Native Pulmonary Surfactant Membranes directly isolated from wild-type and KO protein-D mice bronco-alveolar lavage fluid (BALF). Both mono- and bi-layers show the presence of different structural arrangements, which could indicate a phase lateral coexistence. A closer look at the lipid composition reveals several potential lipidic species which might be playing a role in the segregation phenomena in addition to SP-D. A detailed characterization and correlation with surfactant biophysical functional properties, as well as with particular molecular species is currently being performed. This experimental approach is extremely powerful to correlate the structural, biochemical, and biophysical properties of any compositionally complex material. |
| Databáze: | OpenAIRE |
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