New functions for the components of glucocorticoid receptor apparatus?
| Autor: | A.A. Shnyra, Yu.M. Seleznev, L.A. Medvedeva |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Receptors
Steroid Macromolecular Substances medicine.medical_treatment Size-exclusion chromatography Biophysics Glucocorticoid receptor Biology Biochemistry Triamcinolone Acetonide Steroid Glucocorticoid Cytosol Receptors Glucocorticoid Structural Biology Genetics medicine Animals Receptor Desoxycorticosterone Molecular Biology Gel electrophoresis Myocardium Heart Adrenalectomy Rats Inbred Strains Cell Biology Hormone Rats Molecular Weight Liver Sephadex medicine.drug |
| Zdroj: | FEBS letters. 171(1) |
| ISSN: | 0014-5793 |
| Popis: | By ammonium sulphate precipitation, phosphocellulose and DEAE—Sephacel chromatography and Sephadex G-75 gel filtration, a factor was separated from rat liver cytosol which was shown to suppress the inhibitory effect of the steroid deoxycorticosterone (DOC) on the specific [3H]glucocorticoid binding to cytoplasmic receptors. By SDS-polyacrylamide gel electrophoresis (SDS—PAGE) of factor-containing fractions its Mr was suggested to be about 40000. The possible role of this factor in the regulation of glucocorticoid receptor apparatus function is discussed. |
| Databáze: | OpenAIRE |
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