Redefining the properties of an osmotic agent in an intestinal-specific preservation solution
| Autor: | Angela Ochs, Thomas A. Churchill, Rachel G. Khadaroo, Matthew S. Kokotilo, Aducio Thiesen, Kimberly Schlachter, Jodi M. Carter |
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| Rok vydání: | 2010 |
| Předmět: |
Osmosis
Adenosine Time Factors Allopurinol Organ Preservation Solutions Energy metabolism Cold storage Intestinal absorption Rats sprague dawley Permeability Rats Sprague-Dawley Adenosine Triphosphate Raffinose Intestinal mucosa Intestine Small Animals Insulin Intestinal Mucosa Cold ischemia Chemistry Cold Ischemia Gastroenterology Dextrans General Medicine Glutathione Rats Molecular Weight Oxidative Stress Biochemistry Intestinal Absorption Reperfusion Injury Biophysics Original Article Energy Metabolism Fluorescein-5-isothiocyanate |
| Zdroj: | World journal of gastroenterology. 16(45) |
| ISSN: | 2219-2840 |
| Popis: | To investigate the effects of dextrans of various molecular weights (Mw) during a 12 h cold storage time-course on energetics, histology and mucosal infiltration of fluorescein isothiocyanate (FITC)-dextran.Rodent intestines were isolated and received a standard University of Wisconsin vascular flush followed by intraluminal administration of a nutrient-rich preservation solution containing dextrans of varying Mw: Group D1, 73 kdal; Group D2, 276 kdal; Group D3, 534 kdal; Group D4, 1185 kdal; Group D5, 2400 kdal.Using FITC-labeled dextrans, fluorescent micrographs demonstrated varying degrees of mucosal infiltration; lower Mw (groups D1-D3: 73-534 kdal) dextrans penetrated the mucosa as early as 2 h, whereas the largest dextran (D5: 2400 kdal) remained captive within the lumen and exhibited no permeability even after 12 h. After 12 h, median injury grades ranged from 6.5 to 7.5 in groups D1-D4 (73-1185 kdal) representing injury of the regenerative cryptal regions and submucosa; this was in contrast to group D5 (2400 kdal) which exhibited villus denudation (with intact crypts) corresponding to a median injury grade of 4 (P0.05). Analysis of tissue energetics reflected a strong positive correlation between Mw and adenosine triphosphate (r(2) = 0.809), total adenylates (r(2) = 0.865) and energy charge (r(2) = 0.667).Our data indicate that dextrans of Mw2400 kdal act as true impermeant agents during 12 h ischemic storage when incorporated into an intraluminal preservation solution. |
| Databáze: | OpenAIRE |
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