Patient-specific meniscus prototype based on 3D bioprinting of human cell-laden scaffold
| Autor: | Ugo Albisinni, Giuseppe Filardo, Brunella Grigolo, Carola Cavallo, Stefano Durante, Mauro Petretta, Livia Roseti |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Scaffold
Cell-Laden Bio-Ink 02 engineering and technology Meniscus (anatomy) law.invention 03 medical and health sciences 0302 clinical medicine law medicine Orthopedics and Sports Medicine Meniscus 030222 orthopedics 3D bioprinting Meniscal tissue business.industry Research Mesenchymal stem cell Bioprinting Mesenchymal Stem Cells Human cell Patient specific 021001 nanoscience & nanotechnology medicine.anatomical_structure Surgery 0210 nano-technology business Biomedical engineering |
| Zdroj: | Bone & Joint Research |
| ISSN: | 2046-3758 |
| Popis: | Objectives Meniscal injuries are often associated with an active lifestyle. The damage of meniscal tissue puts young patients at higher risk of undergoing meniscal surgery and, therefore, at higher risk of osteoarthritis. In this study, we undertook proof-of-concept research to develop a cellularized human meniscus by using 3D bioprinting technology. Methods A 3D model of bioengineered medial meniscus tissue was created, based on MRI scans of a human volunteer. The Digital Imaging and Communications in Medicine (DICOM) data from these MRI scans were processed using dedicated software, in order to obtain an STL model of the structure. The chosen 3D Discovery printing tool was a microvalve-based inkjet printhead. Primary mesenchymal stem cells (MSCs) were isolated from bone marrow and embedded in a collagen-based bio-ink before printing. LIVE/DEAD assay was performed on realized cell-laden constructs carrying MSCs in order to evaluate cell distribution and viability. Results This study involved the realization of a human cell-laden collagen meniscus using 3D bioprinting. The meniscus prototype showed the biological potential of this technology to provide an anatomically shaped, patient-specific construct with viable cells on a biocompatible material. Conclusion This paper reports the preliminary findings of the production of a custom-made, cell-laden, collagen-based human meniscus. The prototype described could act as the starting point for future developments of this collagen-based, tissue-engineered structure, which could aid the optimization of implants designed to replace damaged menisci. Cite this article: G. Filardo, M. Petretta, C. Cavallo, L. Roseti, S. Durante, U. Albisinni, B. Grigolo. Patient-specific meniscus prototype based on 3D bioprinting of human cell-laden scaffold. Bone Joint Res 2019;8:101–106. DOI: 10.1302/2046-3758.82.BJR-2018-0134.R1. |
| Databáze: | OpenAIRE |
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