Additional file 3 of Cancer-associated fibroblasts promote oral squamous cell carcinoma progression through LOX-mediated matrix stiffness

Autor: Zhang, Jia-Yi, Zhu, Wei-Wen, Wang, Meng-Yao, Zhai, Run-Dong, Wang, Qiong, Shen, Wei-Li, Liu, Lai-Kui
Rok vydání: 2021
DOI: 10.6084/m9.figshare.17305617
Popis: Additional file 3: Fig. S3. Matrix stiffness increased by drug modification promotes invasion ability of OSCC cells. A, Relative stiffness of collagen modified by huLOX, ribose and BAPN under stable condition was shown. Elastic modulus (G���) were measured by MARS60 microinfrared rheometer. B, Representative immunofluorescence images of F-actin staining in Cal27 cells were shown. Green: F-actin; Blue: DAPI (400 ��). C, The ability of Cal27 cells invaded collagen gels with different stiffness was determined by transwell assay. Representative images of invaded cells and quantification data were shown. D, The protein expression of EMT markers in Cal27 cells cultured on collagen gels were measured by western blot assays. ��-actin served as loading control. The data are presented as the means �� SD; *P
Databáze: OpenAIRE