Spatiotemporal expression pattern of Sjfz7 and its expression comparison with other frizzled family genes in developmental stages of Schistosoma japonicum
| Autor: | Chunxiu Yuan, Hao Li, Ye Zhongxue, Ke Lu, Mingxin Song, Jiaojiao Lin, Xingang Feng, Liqun Wang, Jingxiu Xu, Zhiqiang Fu, Yingying Jia, Yang Hong |
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| Rok vydání: | 2019 |
| Předmět: |
Frizzled
Cell signaling Schistosoma japonicum 03 medical and health sciences 0302 clinical medicine Genetics Animals Amino Acid Sequence Receptor Molecular Biology Gene 030304 developmental biology 0303 health sciences Flatworm Messenger RNA Sequence Homology Amino Acid biology Wnt signaling pathway Gene Expression Regulation Developmental biology.organism_classification Frizzled Receptors Cell biology Schistosomiasis japonica Transcriptome 030217 neurology & neurosurgery Signal Transduction Developmental Biology |
| Zdroj: | Gene Expression Patterns. 32:44-52 |
| ISSN: | 1567-133X |
| DOI: | 10.1016/j.gep.2019.02.005 |
| Popis: | Wnts are secreted signaling molecules that are implicated in a variety of growth-related processes. Frizzled proteins have been identified as receptors for Wnt ligands in vertebrates and invertebrates, but a functional role for dioecious flatworm Frizzleds has not been determined. To evaluate the endogenous role of Frizzled proteins during development, we have identified and characterized a Schistosoma japonicum frizzled gene (Sjfz7). We found that Sjfz7 encodes a 698 amino acid protein with typical characteristics of Frizzled proteins. The immunohistochemical localization pattern showed that Sjfz7 protein was extensively distributed in almost all tissues of S. japonicum, including subtegumental muscle cells, parenchymal cells, intestinal epithelial cells and male and female germ cells. This indicated that Sjfz7-mediated Wnt signaling might be associated with the development of musculature, intestinal tract and reproductive organs in schistosome. Comparing mRNA levels between frizzled family members showed that Sjfz7 mRNA was consistently higher in the developmental stages analyzed, suggesting that Sjfz7 may be responsible for more functional tasks than other frizzled family members. Comparing frizzled mRNA levels between not fully developed and normal worms suggested that Wnt signaling might be abnormal in not fully developed worms. |
| Databáze: | OpenAIRE |
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