Oral immunisation of naive and primed animals with transgenic potato tubers expressing LT-B

Autor: D Bosch, D.E.A Florack, W.J.A Boersma, T.J van der Wal, L.A.Th Hilgers, J.W Molthoff, Tosca G.M. Lauterslager, Jan P. M. Langeveld
Jazyk: angličtina
Rok vydání: 2001
Předmět:
Bacterial Toxins
Immunization
Secondary
Administration
Oral
medicine.disease_cause
Microbiology
Subcutaneous injection
Enterotoxins
Mice
Immune system
Transformation
Genetic
Antigen
Oral administration
Oral booster
Oral immunisation
medicine
Animals
Instituut voor Dierhouderij en Diergezondheid
Solanum tuberosum
General Veterinary
General Immunology and Microbiology
biology
Vaccines
Edible
ID-Lelystad
Toxin
Immunogenicity
Escherichia coli Proteins
fungi
Public Health
Environmental and Occupational Health
food and beverages
Plants
Genetically Modified
Antibodies
Bacterial
Immunoglobulin A
ID Lelystad
Infectious Diseases
LT-B
ID-Lelystad
Instituut voor Dierhouderij en Diergezondheid
Plant Research International
ID Lelystad
Institute for Animal Science and Health
biology.protein
Edible vaccines
Molecular Medicine
Female
Antibody
Patatin
Institute for Animal Science and Health
Zdroj: Vaccine, 19, 2749-2755
Vaccine 19 (2001)
ISSN: 0264-410X
Popis: The efficacy of edible vaccines produced in potato tubers was examined in mice. Transgenic plants were developed by Agrobacterium tumefaciens-mediated transformation. The antigen selected was the non-toxic B subunit of the Escherichia coli enterotoxin (recLT-B). A synthetic gene coding for recLT-B was made and optimised for expression in potato tubers and accumulation in the endoplasmic reticulum. Introduction of this gene under control of the tuber-specific patatin promoter in potato plants resulted in the production of functional, i.e. Gm1-binding, recLT-B pentamers in tubers. Selected tubers containing about 13 microg of recLT-B per gram fresh weight were used for immunisation. Subcutaneous immunisation with an extract of recLT-B tubers yielded high antibody titres in serum that were similar to those obtained with bacterial recLT-B. The efficacy of oral administration of recLT-B tubers was determined by measuring mucosal and systemic immune responses in naive and primed mice. Animals were primed by subcutaneous injection of an extract of recLT-B tuber plus adjuvant. Naive and primed mice were fed 5 g of tubers ( approximately 65 microg of recLT-B) or were intubated intragastrically with 0.4 ml of tuber extract ( approximately 2 microg of recLT-B). In naive mice, feeding recLT-B tubers or intubation of tuber extract did not induce detectable anti-LT antibody titres. In primed animals, however, oral immunisation resulted in significant anti-LT IgA antibody responses in serum and faeces. Intragastric intubation of tuber extract revealed higher responses than feeding of tubers. These results indicate clearly that functional recLT-B can be produced in potato tubers, that this recombinant protein is immunogenic and that oral administration thereof elicits both systemic and local IgA responses in parentally primed, but not naive, animals.
Databáze: OpenAIRE
Externí odkaz: