Role of spinal 5-HT 2 receptors subtypes in formalin-induced long-lasting hypersensitivity
| Autor: | Guadalupe C. Vidal-Cantú, Beatriz Godínez-Chaparro, Vinicio Granados-Soto, Claudia Cervantes-Durán |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Agonist Ketanserin RS-127445 medicine.drug_class RS-102221 Pharmacology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Formaldehyde Ganglia Spinal Animals Medicine Spiro Compounds Rats Wistar Receptor 5-HT receptor Pain Measurement Sulfonamides business.industry General Medicine Rats Pyrimidines 030104 developmental biology Allodynia Spinal Cord chemistry Hyperalgesia Anesthesia Female medicine.symptom Receptors Serotonin 5-HT2 business 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Pharmacological Reports. 68:434-442 |
| ISSN: | 1734-1140 |
| DOI: | 10.1016/j.pharep.2015.11.009 |
| Popis: | Background The purpose of this study was to determine the role of spinal 5-HT 2A , 5-HT 2B and 5-HT 2C receptors in the development and maintenance of formalin-induced long-lasting secondary allodynia and hyperalgesia in rats, as well as their expression in the dorsal root ganglia (DRG) during this process. Methods 0.5–1% formalin was used to produce long-lasting secondary allodynia and hyperalgesia in rats. Western blot was used to determine 5-HT 2 receptors expression in DRG. Results Formalin (0.5–1%) injection produced long-lasting (1–12 days) secondary allodynia and hyperalgesia in both ipsilateral and contralateral hind paws. Intrathecal pre-treatment or post-treatment with the 5-HT 2 receptor agonist, DOI (1–10 nmol), increased 0.5% formalin-induced secondary allodynia and hyperalgesia in both paws. In contrast, intrathecal pre-treatment with the selective 5-HT 2A (ketanserin 1–100 nmol), 5-HT 2B (RS 127445 1–100 nmol) or 5-HT 2C (RS 102221 1–100 nmol) receptor antagonists prevented and reversed, respectively, 1% formalin-induced secondary allodynia and hyperalgesia in both paws. Likewise, the pronociceptive effect of DOI (10 nmol) was blocked by ketanserin, RS 127445 or RS 102221 (0.01 nmol). 5-HT 2A/2B/2C receptors were expressed in DRG of naive rats. Formalin injection (1%) increased bilaterally 5-HT 2A/2B receptors expression in DRG. In contrast, formalin injection decreased 5-HT 2C receptors expression bilaterally in DRG. Conclusion Data suggest that spinal 5-HT 2A/2B/2C receptors have pronociceptive effects and participate in the development and maintenance of formalin-induced long-lasting hypersensitivity. These receptors are expressed in DRG and their expression is modulated by formalin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink