On-chip porous microgel generation for microfluidic enhanced VEGF detection
| Autor: | Zhengtuo Zhao, Mohammad Ali Al-Ameen, Joe F. Lo, Kai Duan, Gargi Ghosh |
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| Rok vydání: | 2015 |
| Předmět: |
Vascular Endothelial Growth Factor A
Materials science VEGF receptors Microfluidics Biomedical Engineering Biophysics Nanotechnology Biosensing Techniques Hydrogel Polyethylene Glycol Dimethacrylate Polyethylene Glycols Limit of Detection Electrochemistry Humans Personalized therapy Porosity Detection limit biology Capture antibody technology industry and agriculture Equipment Design General Medicine Microfluidic Analytical Techniques Flow perfusion biology.protein Antibodies Immobilized Biotechnology Porous hydrogel |
| Zdroj: | Biosensors and Bioelectronics. 74:305-312 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2015.06.047 |
| Popis: | Advances in medical diagnostics and personalized therapy require sensitive and rapid measurement of minute amounts of proteins from patients. Standard ELISA is difficult to prepare and involves lengthy protocols. Here we report a novel method using capture antibody immobilized porous poly (ethylene) glycol diacrylate (PEGDA) hydrogel microspheres to enable high sensitivity VEGF detection in arrayed microfluidics. Our technique incorporates antibody encapsulation, trapping, and flow perfusion on a single device. We showed that the convergence of tunable porous hydrogel with efficient microfluidics improved the sensitivity of the assay. The detection limit of this microfluidic porous microgel based assay was 0.9 pg/mL, with only 1+ hour of assay time, demonstrating a novel assay that exceeded conventional technologies in terms of sensitivity and speed. |
| Databáze: | OpenAIRE |
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