Metabolic and non-metabolic liver zonation is established non-synchronously and requires sinusoidal Wnts

Autor: Ruihua Ma, Beatriz Sosa-Pineda, Angelica Sofia Martínez-Ramírez, Fanding Gao, Thomas L Borders
Rok vydání: 2019
Předmět:
0301 basic medicine
Mouse
Fluorescent Antibody Technique
Gene Expression
Ligands
Mice
0302 clinical medicine
Claudin-2
Biology (General)
Carbon Tetrachloride
hepatic sinusoids
General Neuroscience
Wnt signaling pathway
Age Factors
General Medicine
Immunohistochemistry
Stem Cells and Regenerative Medicine
Cell biology
Liver
030220 oncology & carcinogenesis
Medicine
Stem cell
Signal Transduction
Research Article
Genetically modified mouse
QH301-705.5
Science
CCL4
Mice
Transgenic
Biology
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Wnt
Animals
Secretion
General Immunology and Microbiology
Regeneration (biology)
Endothelial Cells
Capillaries
Wnt Proteins
Disease Models
Animal
030104 developmental biology
Hepatocytes
hepatic zonation
Energy Metabolism
Developmental biology
Homeostasis
Biomarkers
Developmental Biology
Zdroj: eLife
eLife, Vol 9 (2020)
ISSN: 2050-084X
Popis: The distribution of complementary metabolic functions in hepatocytes along a portocentral axis is called liver zonation. Endothelial secreted Wnt ligands maintain metabolic zonation in the adult murine liver but whether those ligands are necessary to initiate zonation in the immature liver has been only partially explored. Also, numerous non-metabolic proteins display zonated expression in the adult liver but it is not entirely clear if their localization requires endothelial Wnts. Here we used a novel transgenic mouse model to compare the spatial distribution of zonated non-metabolic proteins with that of typical zonated metabolic enzymes during liver maturation and after acute injury induced by carbon tetrachloride (CCl4). We also investigated how preventing Wnt ligand secretion from endothelial cells affects zonation patterns under homeostasis and after acute injury. Our study demonstrates that metabolic and non-metabolic zonation are established non-synchronously during maturation and regeneration and require multiple endothelial Wnt sources.
Databáze: OpenAIRE
Externí odkaz: