A new model of chronic peripheral nerve compression for basic research and pharmaceutical drug testing
| Autor: | Lucas Degrugillier, Dirk J. Schaefer, Stefan Schären, Katharina M Prautsch, Daniel F Kalbermatten, Raphael Guzman, Srinivas Madduri |
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| Rok vydání: | 2021 |
| Předmět: |
Embryology
Pathology medicine.medical_specialty business.industry Biomedical Engineering Lumen (anatomy) Sensory system Sciatic Nerve Pathophysiology Nerve conduction velocity Muscle atrophy Axons Nerve Regeneration Rats Rats Sprague-Dawley Electrophysiology medicine.anatomical_structure Pharmaceutical Preparations Peripheral Nerve Injuries Medicine Peripheral nerve compression Animals Axon medicine.symptom business |
| Zdroj: | Regenerative medicine. 16(10) |
| ISSN: | 1746-076X |
| Popis: | Aim: To develop a consistent model to standardize research in the field of chronic peripheral nerve neuropathy. Methods: The left sciatic nerve of 8-week-old Sprague–Dawley rats was compressed using a customized instrument leaving a defined post injury nerve lumen (400 μm, 250 μm, 100 μm, 0 μm) for 6 weeks. Sensory and motor outcomes were measured weekly, and histomorphology and electrophysiology after 6 weeks. Results: The findings demonstrated compression depth-dependent sensory and motor pathologies. Quantitative measurements revealed a significant myelin degeneration, axon irregularities and muscle atrophy. At the functional level, we highlighted the dynamics of the different injury profiles. Conclusion: Our novel model of chronic peripheral nerve compression is a useful tool for research on pathophysiology and new therapeutic approaches. |
| Databáze: | OpenAIRE |
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