Differential expression and analysis of extrachromosomal circular DNAs as serum biomarkers in lung adenocarcinoma
| Autor: | Gang Xu, Wenjing Shi, Liqun Ling, Changhong Li, Fanggui Shao, Jie Chen, Yumin Wang |
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| Rok vydání: | 2022 |
| Předmět: |
Microbiology (medical)
Lung Neoplasms Ubiquitin-Protein Ligases Biochemistry (medical) Clinical Biochemistry Public Health Environmental and Occupational Health Adenocarcinoma of Lung Hematology DNA Receptors G-Protein-Coupled Medical Laboratory Technology Antigens Neoplasm Biomarkers Tumor Immunology and Allergy Humans DNA Circular Apoptosis Regulatory Proteins Co-Repressor Proteins |
| Zdroj: | Journal of clinical laboratory analysis. 36(6) |
| ISSN: | 1098-2825 |
| Popis: | Extrachromosomal circular DNAs (eccDNAs) increase the number of proto-oncogenes by enhancing oncogene expression to promote tumorigenesis. However, there are limited reports on differential eccDNA expression and analysis in lung cancer, especially in lung adenocarcinoma (LAD).Three LAD and three corresponding NT tissues samples were used for eccDNA next-generation sequencing analysis, and an additional 20 were used for quantitative PCR (qPCR) evaluations. We further performed qPCR amplification using serum samples from LAD patients and healthy medical examiners.eccDNAs from LAD samples were mainly 200-1000 bp in length. Gene annotation analysis revealed that most eccDNAs were derived from chromosomes 1 and 2. The top-ten increased and top-ten decreased eccDNAs in LAD tissues were CircD-ARPC1B, CircD-ARPC1A, CircD-FAM49B, CircD-SDK1, CircD-KCNG1, CircD-POLR2F, CircD-SS18L1, CircD-SLC16A3, CircD-CSNK1D, CircD-KCTD1, and CircD-TMIGD2, CircD-PDIA5, CircD-VAV2, CircD-GATAD2A, CircD-CAB39L, CircD-KHDC1, CircD-FOXN3, CircD-SULT2B1, CircD-DPP9, and CircD-CSNK1D. qPCR demonstrated that the expression of CircD-DZRN3 was higher in LAD tissues than in normal lung tissues, whereas CircD-LGR6 and CircD-UMODL1 expression levels were lower in LAD than in normal lung tissues. Furthermore, the serum CircD-PDZRN3 level increased, while CircD-LGR6 decreased in LAD. Receiver operating characteristic (ROC) analysis showed that area under curve (AUC) of serum CircD-PDZRN3 (0.991), CircD-LGR6 (0.916) was higher than that of serum carcinoembryonic antigen (CEA) (0.825), CY211 (cytokeratin 19 fragment) (0.842), SCCA(squamous cell carcinoma antigen) (0.857) for the diagnosis of LAD.Our study first showed that several eccDNAs were aberrantly expressed in LAD, among which CircD-PDZRN3 and CircD-LGR6 clearly distinguished LAD patients from healthy controls, indicating their potential as biomarkers. |
| Databáze: | OpenAIRE |
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