Identification of differential gene expression between intestinal and diffuse gastric cancer using cDNA microarray
| Autor: | Tsann-Long Hwang, En-Shih Chen, Min-Li Wei, Tzu-Hao Wang, Li-Yu Lee, Yun-Shien Lee, Wei-Hsiang Kong, Chi-Ming Wu, Ying Liang |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Cancer Research Microarray Biology CCL7 Stomach Neoplasms Transcription (biology) Complementary DNA Intestinal Neoplasms Gene expression Humans Gene Aged Oligonucleotide Array Sequence Analysis Aged 80 and over Genetics Oncogene Gene Expression Profiling General Medicine Middle Aged Immunohistochemistry Molecular biology Neoplasm Proteins Gene Expression Regulation Neoplastic Gene expression profiling Oncology Female Genes Neoplasm |
| Zdroj: | Oncology Reports. |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.15.1.57 |
| Popis: | To compare the gene expression profiling between intestinal-type gastric cancer (IGC) and diffuse-type gastric cancer (DGC), cDNA microarray containing 7334 gene elements was performed on 12 paired IGC specimens/its' normal epithelial tissue and 11 paired DGC specimens/its' normal epithelial tissue. Twenty-seven genes were co-over-expressed in IGC and DGC. These overexpressed genes were related to transcription and translation, DNA replications and mitosis, calcium binding, apoptosis and mitochondria protein. Twelve genes were co-underexpressed in IGC and DGC. These underexpressed genes were associated with cell adhesion and migration, organelle movement and intracellular transport, and matrix metalloproteinase. A clustering dendrogram of IGC and DGC with 27 genes significantly differed between IGC and DGC. Nineteen genes were more overexpressed in DGC than in IGC, including annexin Al (ANXA1), chemokine ligand 7 (CCL7), and chemokine ligand 8 (CCL8). Eight genes were more overexpressed in IGC than in DGC, including claudin 4 (CLDN4). The results of quantitative real-time PCR and immunohistochemical staining confirmed the microarray finding. The gene expression profiling between IGC and DGC suggested that they might have unique genetic pathways which share some of the same and some different genetic alterations. |
| Databáze: | OpenAIRE |
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