Active vitamin D and vitamin D analogs stimulate fibroblast growth factor 23 production in osteocyte-like cells via the vitamin D receptor
| Autor: | Mitsuru Yashiro, Tomohiro Sonou, Takurou Yano, Toru Mima, Kazuki Kawakami, Kouichi Tatsuta, Sou Kobayashi, Yusuke Tanaka, Yuri Nakashima, Shuto Yamamoto, Takashi Shigematsu, Shigeo Negi, Masaki Ohya |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Paricalcitol
Vitamin Fibroblast growth factor 23 medicine.medical_specialty Clinical Biochemistry Pharmaceutical Science urologic and male genital diseases Osteocytes 01 natural sciences Calcitriol receptor Cell Line Analytical Chemistry Mice chemistry.chemical_compound Internal medicine Drug Discovery medicine Vitamin D and neurology Animals Vitamin D Spectroscopy 010405 organic chemistry Chemistry 010401 analytical chemistry Cell Differentiation Eldecalcitol Ascorbic acid 0104 chemical sciences Fibroblast Growth Factors Fibroblast Growth Factor-23 stomatognathic diseases medicine.anatomical_structure Endocrinology Gene Expression Regulation Gene Knockdown Techniques Osteocyte Receptors Calcitriol medicine.drug |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 182:113139 |
| ISSN: | 0731-7085 |
| Popis: | Osteocytes play an important role in the regulation of serum phosphorus by producing fibroblast growth factor 23 (FGF23). FGF23 production is stimulated by 1α,25-dihydroxyvitamin D in osteocytes. However, it is unclear whether vitamin D induces FGF23 production in osteocytes directly. Therefore, we investigated vitamin D-induced FGF23 production in osteocyte-like cells derived from MC3T3-E1 osteocyte progenitor cells. We also investigated differences in the induction of FGF23 by 1α,25-dihydroxyvitamin D and various vitamin D analogs. MC3T3-E1 cells were differentiated into osteocyte-like cells (MCT3-E1-OLCs) by treatment with various agents including β-glycerophosphate and ascorbic acid. MCT3-E1-OLCs were stimulated with 1α,25-dihydroxyvitamin D3 and subsequent FGF23 gene expression was 2631 ± 605 times higher compared with untreated cells. The expression of FGF23 in MCT3-E1-OLCs transfected with a knockdown sequence against vitamin D receptor (VDR) was significantly decreased compared with that in cells transfected with the control vector. Therefore, the induction of FGF23 in osteocytes by vitamin D may be primarily mediated via VDR. The potential of 25(OH)vitamin D3, paricalcitol, and maxacalcitol to induce FGF23 production was almost the same as that of 1α,25-dihydroxyvitamin D3. However, falecalcitriol and eldecalcitol demonstrated a reduced potential to induce FGF23 compared with 1α,25-dihydroxyvitamin D3. Our results demonstrate that FGF23 induction is different among the analogs of 1α,25-dihydroxyvitamin D3. Therefore, an appropriate vitamin D analog should be chosen for each patient with mineral and bone disorder, considering its effect on FGF23 production. |
| Databáze: | OpenAIRE |
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