The collagenase model of intracerebral hemorrhage in awake, freely moving animals: The effects of isoflurane
| Autor: | Ana C Klahr, D. Ryan Maisey, Cassandra M. Wilkinson, Anna C J Kalisvaart, Clayton T. Dickson, Tiffany F. C. Kung, Frederick Colbourne |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Blood Glucose Male Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Hematoma Seizures Weight Loss medicine Animals cardiovascular diseases Collagenases Molecular Biology Stroke Cerebral Hemorrhage Intracerebral hemorrhage Isoflurane business.industry General Neuroscience Electroencephalography medicine.disease Cannula Rats Burst suppression Disease Models Animal 030104 developmental biology Blood pressure Anesthesia Anesthetic Neurology (clinical) business 030217 neurology & neurosurgery Developmental Biology medicine.drug |
| Zdroj: | Brain research. 1728 |
| ISSN: | 1872-6240 |
| Popis: | Intracerebral hemorrhage (ICH) is a devastating stroke often modelled in rats. Isoflurane anesthetic, commonly used in preclinical research, affects general physiology (e.g., blood pressure) and electrophysiology (e.g., burst suppression) in many ways. These physiological changes may detract from the clinical relevance of the model. Here, we revised the standard collagenase model to produce an ICH in rats without anesthetic. Guide cannulas were implanted stereotaxically under anesthetic. After 3 days of recovery, collagenase was infused through an internal cannula into the striatum of animals randomly assigned to the non-anesthetized or isoflurane group. We assessed whether isoflurane affected hematoma volume, core temperature, movement activity, pain, blood pressure, and seizure activity. With a small ICH, there was a hematoma volume increased from 8.6 (±3.3, 95% confidence interval) µL in anesthetized rats to 13.2 (±3.1) µL in non-anesthetized rats (P = 0.008), but with a larger ICH, hematoma volumes were similar. Isoflurane decreased temperature by 1.3 °C (±0.16 °C, P 0.001) for 2 h and caused a 35.1 (±1.7) mmHg group difference in blood pressure (P 0.007) for 12 m. Blood glucose increased twofold after isoflurane procedures (P 0.001). Pain, as assessed with the rat grimace scale, did not differ between groups. Seizure incidence rate (62.5%) in non-anesthetized ICH rats was similar to historic amounts (61.3%). In conclusion, isoflurane appears to have some significant and injury size-dependent effects on the collagenase model. Thus, when anesthetic effects are a known concern, the use of the standardized cannula infusion approach is scientifically and ethically acceptable. |
| Databáze: | OpenAIRE |
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