Absolute quantitative PCR for detection of molecular biomarkers in melanoma patients: A preliminary report

Autor: Mm Lavieri, Chiara Autilio, Francesca Vendittelli, Ettore Capoluongo, Rodolfo Capizzi, Carmela Paolillo, Sl Silveri
Přispěvatelé: Vendittelli, F., Paolillo, C., Autilio, C., Lavieri, M. M., Silveri, S. L., Capizzi, R., Capoluongo, E.
Rok vydání: 2015
Předmět:
Adult
Male
Oncology
medicine.medical_specialty
Pathology
ATP Binding Cassette Transporter
Subfamily B
Skin Neoplasms
Clinical Biochemistry
CD146 Antigen
Real-Time Polymerase Chain Reaction
Biochemistry
Transforming Growth Factor beta2
Circulating tumor cell
Absolute qRT-PCR
Preliminary report
Cell Line
Tumor
Internal medicine
Gene expression
Biomarkers
Tumor
medicine
Humans
Paired Box Transcription Factors
ATP Binding Cassette Transporter
Subfamily B
Member 1
Multimarker assay
Melanoma
PAX3 Transcription Factor
P-glycoprotein
biology
business.industry
Biochemistry (medical)
Circulating tumor cells
ABCB5
Melanoma molecular biomarker
General Medicine
Middle Aged
medicine.disease
Phenotype
Real-time polymerase chain reaction
biology.protein
Female
Settore MED/35 - MALATTIE CUTANEE E VENEREE
business
Zdroj: Clinica Chimica Acta. 444:242-249
ISSN: 0009-8981
DOI: 10.1016/j.cca.2015.02.013
Popis: Background Malignant melanoma is the most malignant tumours of skin and mucous membranes mainly due to its aggressive biological behaviour and tendency to generate early metastases. Unfortunately, the mechanisms underlying the development, progression and the expression of an aggressive melanoma phenotype still remain largely unknown. Objectives The purpose of this study was to determine whether a multi-panel of molecular transcripts can be predictive for risk of recurrent disease in malignant melanoma patients. Results Peripheral blood was collected from 31 malignant melanoma patients in follow-up for melanoma and from 30 healthy volunteers randomly selected. Each specimen was examined by qRT-PCR analysis for the expression of six markers: PAX3d, TYR, MITFm, MCAM, TGFβ2 and ABCB5. Malignant melanoma patients expressed an important number of markers, with a median value of four markers. Only PAX3d displayed a trend in terms of differences when the levels of gene expression were made in function of Breslow index. Furthermore, PAX3d showed the best diagnostic capacity among the remaining residual markers or in combination with TGFβ2 and MTIF. Conclusions We demonstrated the usefulness of multimarker qRT-PCR to detect circulating melanoma cells in blood and to potentially assessing patient disease status or progression, especially when PAX3d was used in combination with MTIFm and TGFβ2.
Databáze: OpenAIRE
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