GnRH agonist decreases endothelial nitric oxide synthase (eNOS) expression in leiomyoma
| Autor: | Bulent Mizrak, N. Bazoglu, Suleyman Ozen, Remzi Gokdeniz |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Agonist medicine.medical_specialty Antineoplastic Agents Hormonal Nitric Oxide Synthase Type III Endothelium medicine.drug_class Nitric oxide Gonadotropin-Releasing Hormone chemistry.chemical_compound Enos Internal medicine medicine Humans Myocyte Leiomyoma biology business.industry Myometrium Estrogen secretion Obstetrics and Gynecology General Medicine musculoskeletal system medicine.disease biology.organism_classification Immunohistochemistry female genital diseases and pregnancy complications medicine.anatomical_structure Endocrinology chemistry Uterine Neoplasms Female Nitric Oxide Synthase business |
| Zdroj: | International Journal of Gynecology & Obstetrics. 70:347-352 |
| ISSN: | 0020-7292 |
| DOI: | 10.1016/s0020-7292(00)00198-3 |
| Popis: | Objective: To define the effect of GnRH agonist (GnRHa) treatment on endothelial nitric oxide synthase (eNOS) expression in leiomyoma. As eNOS expression is more pronounced in leiomyoma compared to parental myometrium, we hypothesized that the mechanism(s) of tumor shrinkage by GnRHa may be due to decreased nitric oxide (NO) production in leiomyoma. Methods: Eleven patients with leiomyoma were operated for myoma enucleation by laparatomy. Six of them were treated with GnRHa every 28 days, three times before the operation. The remaining five patients who had no treatment prior to operation formed the control group. Blood was drawn from the patients before treatment and on the day of operation for the assay of serum estradiol (E2). Immunohistochemical localization of eNOS expression in leiomyoma and myometrium in treated patients, and in leiomyoma in the control group, was performed using monoclonal antibodies specific to eNOS. Results: All treated subjects showed a significant reduction of fibroid volume at the end of therapy. eNOS-positive cells were localized primarily within the vascular endothelium and smooth muscle cells, but had weak expression in fibroid and myometrial muscle cells in the treated group. The immunoreactivity was similar for both the leiomyoma and myometrium (P>0.05). In contrast to this, the control group had shown strong expression in leiomyoma muscle cells (P |
| Databáze: | OpenAIRE |
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