Asiatic Acid Protects Dopaminergic Neurons from Neuroinflammation by Suppressing Mitochondrial ROS Production
| Autor: | Wei-Xiong Chen, Jing Gao, Xiao-Ya Zhang, Jin-Jun Qian, Jing Sun, Dong Chen, Qi-Jie Cong, Hafiz Muhammad Ahsan |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Mitochondrial ROS Mitochondrion Asiatic acid Biochemistry Neuroprotection 03 medical and health sciences 0302 clinical medicine Neuroinflammation Drug Discovery medicine Pharmacology chemistry.chemical_classification Reactive oxygen species Microglia Dopaminergic Inflammasome NLRP3 inflammasome Mitochondria Cell biology 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Parkinson’s disease Molecular Medicine Original Article medicine.drug |
| Zdroj: | Biomolecules & Therapeutics |
| ISSN: | 2005-4483 1976-9148 |
| DOI: | 10.4062/biomolther.2018.188 |
| Popis: | This study sought to evaluate the effects of Asiatic acid in LPS-induced BV2 microglia cells and 1-methyl-4-phenyl-pyridine (MPP+)-induced SH-SY5Y cells, to investigate the potential anti-inflammatory mechanisms of Asiatic acid in Parkinson’s disease (PD). SH-SY5Y cells were induced using MPP+ to establish as an in vitro model of PD, so that the effects of Asiatic acid on dopaminergic neurons could be examined. The NLRP3 inflammasome was activated in BV2 microglia cells to explore potential mechanisms for the neuroprotective effects of Asiatic acid. We showed that Asiatic acid reduced intracellular production of mitochondrial reactive oxygen species and altered the mitochondrial membrane potential to regulate mitochondrial dysfunction, and suppressed the NLRP3 inflammasome in microglia cells. We additionally found that treatment with Asiatic acid directly improved SH-SY5Y cell viability and mitochondrial dysfunction induced by MPP+. These data demonstrate that Asiatic acid both inhibits the activation of the NLRP3 inflammasome by downregulating mitochondrial reactive oxygen species directly to protect dopaminergic neurons from, and improves mitochondrial dysfunction in SH-SY5Y cells, which were established as a model of Parkinson’s disease. Our finding reveals that Asiatic acid protects dopaminergic neurons from neuroinflammation by suppressing NLRP3 inflammasome activation in microglia cells as well as protecting dopaminergic neurons directly. This suggests a promising clinical use of Asiatic acid for PD therapy. |
| Databáze: | OpenAIRE |
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