Enhancedin vivovisualization of the microcirculation by topical application of fructose solution confirmed with correlation mapping optical coherence tomography
| Autor: | Martin J. Leahy, Susan M. Daly, James McGrath, Joey Enfield |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male Materials science genetic structures Administration Topical Biomedical Engineering Image processing Fructose 02 engineering and technology 01 natural sciences Microcirculation 010309 optics Biomaterials Optics Optical coherence tomography 0103 physical sciences Image Processing Computer-Assisted medicine Humans Penetration depth Ballistic photon Skin medicine.diagnostic_test Phantoms Imaging business.industry 021001 nanoscience & nanotechnology eye diseases Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Visualization sense organs Tomography 0210 nano-technology business Tomography Optical Coherence Preclinical imaging |
| Zdroj: | Journal of Biomedical Optics. 21:081212 |
| ISSN: | 1083-3668 |
| DOI: | 10.1117/1.jbo.21.8.081212 |
| Popis: | Changes within the microcirculation can provide an early indication of the onset of a plethora of ailments. Various techniques have thus been developed that enable the study of microcirculatory irregularities. Correlation mapping optical coherence tomography (cmOCT) is a recently proposed technique, which enables mapping of vasculature networks at the capillary level in a noninvasive and noncontact manner. This technique is an extension of conventional optical coherence tomography (OCT) and is therefore likewise limited in the penetration depth of ballistic photons in biological media. Optical clearing has previously been demonstrated to enhance the penetration depth and the imaging capabilities of OCT. In order to enhance the achievable maximum imaging depth, we propose the use of optical clearing in conjunction with the cmOCT technique. We demonstrate in vivo a 13% increase in OCT penetration depth by topical application of a high-concentration fructose solution, thereby enabling the visualization of vessel features at deeper depths within the tissue. |
| Databáze: | OpenAIRE |
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