Isolation of a sequence which maps close to the human sex determining gene
| Autor: | P.N. Goodfellow, Paul J. Goodfellow, C.A. Pritchard |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Genetics
Chromosome 7 (human) Sex Determination Analysis Base Sequence Gene map Nucleic acid sequence Chromosome Mapping Nucleic Acid Hybridization Locus (genetics) Biology Transfection Y chromosome Chromosome 17 (human) Sequence Homology Nucleic Acid Y Chromosome Humans Chromosome Deletion Chromosome 22 Southern blot |
| Zdroj: | Nucleic Acids Research. 15:6159-6169 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/15.15.6159 |
| Popis: | A sequence mapping close to the human sex determining gene (TDF) has been isolated from a lambda library constructed with DNA derived from a chromosome transfectant hybrid cell line. This sequence is shown to be present in the DNA of X-Y interchange males at a very high frequency and, based on these studies, it is categorised with the sequence defined by the probe, GMGY3, as the closest known Y chromosome derived marker to TDF. In contrast to GMGY3, however, this locus shares no homology with any other human chromosome. Southern blot analysis also reveals specific hybridization to the Y chromosome of other primates. It therefore defines, for the first time, a conserved and Y chromosome unique locus that is near to TDF. |
| Databáze: | OpenAIRE |
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