[Measurement of urinary epidermal growth factor by radioimmunoassay and its application]
| Autor: | Mizuno I, Fuse H, Takashi Katayama, Horiuchi J, Kurimoto F |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Adult
Male medicine.medical_specialty Urinary system Radioimmunoassay Urine Peptide hormone Sensitivity and Specificity chemistry.chemical_compound Epidermal growth factor Reference Values Internal medicine medicine Humans Aged Aged 80 and over Creatinine biology Epidermal Growth Factor Prostatic Neoplasms Reproducibility of Results Middle Aged Kidney Neoplasms Endocrinology chemistry Urinary Bladder Neoplasms biology.protein Female Antibody Hormone |
| Zdroj: | Nihon Naibunpi Gakkai zasshi. 67(5) |
| ISSN: | 0029-0661 |
| Popis: | Epidermal growth factor (EGF), a polypeptide hormone originally discovered in the mouse submaxillary gland, stimulates growth in a variety of tissues in several species. This hormone has recently been identified in human urine. Herein, radioimmunoassay (RIA) for human EGF (hEGF) has been developed, using human recombinant EGF as reference standard and radioiodinated tracer and antibodies raised against hEGF. Its sensitivity was somewhat higher than that of RIAs reported formerly. This method had no cross reactivity with high molecular weight EGF. Urinary concentrations of hEGF in normal males and females were 30.2 +/- 13.0 ng/mg creatinine and 46.4 +/- 17.4 ng/mg creatinine, respectively. Urinary concentration of hEGF in patients with renal cancer was somewhat low. The patients with bladder cancer had lower urinary excretion of hEGF than normal adults (p less than 0.05). However, the role of hEGF in health and disease remains to be determined. |
| Databáze: | OpenAIRE |
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