Waterproof Galvanometer Scanner-Based Handheld Photoacoustic Microscopy Probe for Wide-Field Vasculature Imaging In Vivo
| Autor: | Sangyeob Han, Jaeyul Lee, Mansik Jeon, Euimin Lee, Jeehyun Kim, Junsoo Lee, Daewoon Seong, Yoonseok Kim |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
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Materials science handheld probe 02 engineering and technology 01 natural sciences Imaging phantom 010309 optics symbols.namesake In vivo 3D imaging 0103 physical sciences Radiology Nuclear Medicine and imaging Applied optics. Photonics wide-field imaging Instrumentation Image resolution in vivo vasculature imaging Resolution (electron density) photoacoustic microscopy 021001 nanoscience & nanotechnology Galvanometer Atomic and Molecular Physics and Optics TA1501-1820 symbols IRIS (biosensor) 0210 nano-technology Preclinical imaging Biomedical engineering |
| Zdroj: | Photonics, Vol 8, Iss 305, p 305 (2021) |
| ISSN: | 2304-6732 |
| Popis: | Photoacoustic imaging (PAI) is a hybrid non-invasive imaging technique used to merge high optical contrast and high acoustic resolution in deep tissue. PAI has been extensively developed by utilizing its advantages that include deep imaging depth, high resolution, and label-free imaging. As a representative implementation of PAI, photoacoustic microscopy (PAM) has been used in preclinical and clinical studies for its micron-scale spatial resolution capability with high optical absorption contrast. Several handheld and portable PAM systems have been developed that improve its applicability to several fields, making it versatile. In this study, we developed a laboratory-customized, two-axis, waterproof, galvanometer scanner-based handheld PAM (WP-GVS-HH-PAM), which provides an extended field of view (14.5 × 9 mm2) for wide-range imaging. The fully waterproof handheld probe enables free movement for imaging regardless of sample shape, and volume rate and scanning region are adjustable per experimental conditions. Results of WP-GVS-HH-PAM-based phantom and in vivo imaging of mouse tissues (ear, iris, and brain) confirm the feasibility and applicability of our system as an imaging modality for various biomedical applications. |
| Databáze: | OpenAIRE |
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