Accumulation of two alkylating agents, nitrogen mustard and busulfan, by murine leukemia cells in vitro
| Autor: | Isidore Wodinsky, Marjorie Myers, David Kessel |
|---|---|
| Rok vydání: | 1969 |
| Předmět: |
medicine.medical_treatment
In Vitro Techniques Biology Biochemistry chemistry.chemical_compound Nucleic Acids medicine Animals Neoplasm Mechlorethamine Amines Leukemia L1210 Uracil Busulfan Pharmacology Chemotherapy Leukemia Experimental Temperature Metabolism medicine.disease In vitro Nitrogen mustard Leukemia chemistry Nucleic acid Protein Binding Thymidine medicine.drug |
| Zdroj: | Biochemical Pharmacology. 18:1229-1234 |
| ISSN: | 0006-2952 |
| DOI: | 10.1016/0006-2952(69)90126-9 |
| Popis: | The accumulation of two alkylating agents by murine leukemia cells in vitro was studied. Nitrogen mustard (HN 2 ) and busulfan rapidly penetrated cells; cell-medium drug distribution ratios of one were established within 3 min at 0° over a wide range of external drug levels. Accumulation of busulfan was not much affected by raising the incubation temperature. NH 2 binding was, however, a remarkably temperature-sensitive process. At10 −5 M levels, busulfan and HN 2 partly inhibited cellular incorporation of precursors into nucleic acids in vitro . When cell types differing widely in sensitivity to these alkylating agents were tested, no differences in capacity for drug accumulation or in subsequent inhibition of nucleic acid synthesis could be found. |
| Databáze: | OpenAIRE |
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