Phosphate transport in Arabidopsis: Pht1;1 and Pht1;4 play a major role in phosphate acquisition from both low- and high-phosphate environments
| Autor: | Heungsop Shin, Maria J. Harrison, Gary R. Dewbre, Hwa-Soo Shin |
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| Rok vydání: | 2004 |
| Předmět: |
Mutant
Arabidopsis chemistry.chemical_element Gene Expression Plant Science Biology Plant Roots Phosphates chemistry.chemical_compound Gene Expression Regulation Plant Genetics Arabidopsis thaliana Gene Regulation of gene expression Arabidopsis Proteins Phosphorus Wild type Biological Transport Cell Biology Phosphate biology.organism_classification chemistry Biochemistry Mutation Genome Plant Signal Transduction |
| Zdroj: | The Plant journal : for cell and molecular biology. 39(4) |
| ISSN: | 0960-7412 |
| Popis: | Of the mineral nutrients essential for plant growth, phosphorus plays the widest diversity of roles and a lack of phosphorus has profound effects on cellular metabolism. At least eight members of the Arabidopsis Pht1 phosphate (Pi) transporter family are expressed in roots and Pht1;1 and Pht1;4 show the highest transcript levels. The spatial and temporal expression patterns of these two genes show extensive overlap. To elucidate the in planta roles of Pht1;1 and Pht1;4, we identified loss-of-function mutants and also created a double mutant, lacking both Pht1;1 and Pht1;4. Consistent with their spatial expression patterns, membrane location and designation as high-affinity Pi transporters, Pht1;1 and Pht1;4 contribute to Pi transport in roots during growth under low-Pi conditions. In addition, during growth under high-Pi conditions, the double mutant shows a 75% reduction in Pi uptake capacity relative to wildtype. Thus, Pht1;1 and Pht1;4 play significant roles in Pi acquisition from both low- and high-Pi environments. |
| Databáze: | OpenAIRE |
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