Immortalization of Normal Adult Human Middle Ear Epithelial Cells Using a Retrovirus Containing the E6/E7 Genes of Human Papillomavirus Type 16
| Autor: | Young-Myoung Chun, Derald E. Brackmann, Johng S. Rhim, Sung-Kyun Moon, David J. Lim, Haa-Yung Lee, Paul Webster |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
Papillomavirus E7 Proteins Genetic Vectors Ear Middle Mice Nude Virus Cell Line Mice 03 medical and health sciences 0302 clinical medicine Retrovirus medicine Animals Humans 030223 otorhinolaryngology Papillomaviridae biology Reverse Transcriptase Polymerase Chain Reaction Epithelial Cells Oncogene Proteins Viral General Medicine biology.organism_classification Phenotype Virology Epithelium Repressor Proteins medicine.anatomical_structure Otitis Otorhinolaryngology Cell culture 030220 oncology & carcinogenesis Middle ear Cancer research medicine.symptom |
| Zdroj: | Annals of Otology, Rhinology & Laryngology. 111:507-517 |
| ISSN: | 1943-572X 0003-4894 |
| DOI: | 10.1177/000348940211100606 |
| Popis: | A human middle ear epithelial cell line (HMEEC-1) was established using human papillomavirus E6/E7 genes. HMEEC-1 has remained morphologically and phenotypically stable, even after 50 passages. The cells are anchorage-dependent and nontumorigenic when injected into nude mice. This cell line thus provides a new tool for the study of normal cell biology and the pathological processes associated with the epithelial cells of the middle ear in otitis media. HMEEC-1 will also be useful in the search for new drugs and biological agents for the treatment of otitis media. |
| Databáze: | OpenAIRE |
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