Switching of cardiac troponin I between nuclear and cytoplasmic localization during muscle differentiation
| Autor: | Eugene A. Arifulin, Yana R. Musinova, Denis Nalobin, Eugene V. Sheval, Maria Y. Shubina, Georgii A. Nosov, Olga M. Lisitsyna, Alexey V. Kharitonov, Anastasia V. Mamontova |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
alpha Karyopherins
Cytoplasm Nuclear Localization Signals Active Transport Cell Nucleus Myoblasts 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Troponin I medicine Animals Humans Myocyte Amino Acid Sequence Molecular Biology 030304 developmental biology Cell Nucleus 0303 health sciences Microscopy Confocal Chemistry Cell Differentiation Cell Biology beta Karyopherins Cell biology Cytosol medicine.anatomical_structure 030220 oncology & carcinogenesis Mutagenesis Site-Directed Nuclear transport Myofibril Sequence Alignment Nucleus Nuclear localization sequence |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1867:118601 |
| ISSN: | 0167-4889 |
| DOI: | 10.1016/j.bbamcr.2019.118601 |
| Popis: | The nuclear accumulation of proteins may depend on the presence of short targeting sequences, which are known as nuclear localization signals (NLSs). Here, we found that NLSs are predicted in some cytosolic proteins and examined the hypothesis that these NLSs may be functional under certain conditions. As a model, human cardiac troponin I (hcTnI) was used. After expression in cultured non-muscle or undifferentiated muscle cells, hcTnI accumulated inside nuclei. Several NLSs were predicted and confirmed by site-directed mutagenesis in hcTnI. Nuclear import occurred via the classical karyopherin-α/β nuclear import pathway. However, hcTnI expressed in cultured myoblasts redistributed from the nucleus to the cytoplasm, where it was integrated into forming myofibrils after the induction of muscle differentiation. It appears that the dynamic retention of proteins inside cytoplasmic structures can lead to switching between nuclear and cytoplasmic localization. |
| Databáze: | OpenAIRE |
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