Nucleotide sequence of an Escherichia coli tRNA (Leu 1) operon and identification of the transcription promoter signal
| Autor: | W. Michael Holmes, Renee K. Campen, Gregg Duester |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Genetics
Base Sequence Transcription Genetic Operon Base pair Nucleic acid sequence Nucleic Acid Hybridization DNA DNA Restriction Enzymes RNA Transfer Amino Acyl Biology Molecular biology Deoxyribonuclease EcoRI Nucleic acid thermodynamics Plasmid Transfer RNA Escherichia coli Pribnow box Cloning Molecular Gene Plasmids |
| Zdroj: | Nucleic Acids Research. 9:2121-2140 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/9.9.2121 |
| Popis: | Fourteen different DNA fragments containing Escherichia coli tRNA genes have been cloned using the vector pBR322. We report the methods of cloning, the identification of specific tRNA genes, and the presence or absence of rRNA genes on these cloned DNA fragments. In particular, one chimeric plasmid contains a 17.0 kilobase pair EcoRI fragment bearing tRNA(Leu 1) sequences. Using nucleotide sequence analysis we have identified a cluster of three tandem tRNA(Leu 1) genes separated by intergenic spacers of 27 and 34 base pairs, respectively. The nucleotide sequence upstream of the first gene contains a transcription promoter site. A G-C rich sequence (5'-CGCCTCC-3') found between the Pribnow box and initiation site is very similar to the corresponding sequence found in other genes which are under stringent control. |
| Databáze: | OpenAIRE |
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