Short monolithic columns—A breakthrough in purification and fast quantification of tomato mosaic virus
| Autor: | Maja Ravnikar, Aleš Štrancar, Matjaž Peterka, Jana Boben, Petra Kramberger |
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| Rok vydání: | 2007 |
| Předmět: |
Virus quantification
Time Factors Monolithic HPLC column Chromatography Downstream processing biology Chemistry Organic Chemistry Ion chromatography Reproducibility of Results Tobamovirus General Medicine Chromatography Ion Exchange biology.organism_classification Biochemistry Analytical Chemistry Tobacco Mosaic Virus Tobacco mosaic virus Electrophoresis Polyacrylamide Gel Tomato mosaic virus Purification methods |
| Zdroj: | Journal of Chromatography A. 1144:143-149 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2006.10.055 |
| Popis: | Drawbacks of conventional virus purification methods have led to the development of new, mostly chromatography-based methods. Short monolithic columns are stationary phases intended for purification of large molecules. In this work efficient chromatographic purification of tomato mosaic virus (ToMV) from plant material is described. Based on short monolithic column, the purification process was shortened from 5 days to 2 hours. High viral purity was achieved and recovery of chromatographic step was up to 90%. In addition, these columns enabled preliminary quantification of the virus in just a few minutes, much faster than other quantification methods (e.g. enzyme-linked immunosorbent assay or real-time polymerase chain reaction) which take 1-2 days. These results demonstrate the potential of short monolith column technology for purification and analysis of different viruses. |
| Databáze: | OpenAIRE |
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