Photo-immobilization of proteins on carbons
| Autor: | Moisés Canle, Cristiane Nunes Lopes, Nito Angelo Debacher, Regina de Fátima Peralta Muniz Moreira, M. Isabel Fernández Pérez, Eduardo Humeres, Vladimir Safin, J. Arturo Santaballa |
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| Rok vydání: | 2019 |
| Předmět: |
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Biophysics Oxide chemistry.chemical_element law.invention chemistry.chemical_compound law Animals Radiology Nuclear Medicine and imaging Bovine serum albumin Deoxygenation Radiation Photolysis Radiological and Ultrasound Technology biology Graphene Photodissociation Water Serum Albumin Bovine Enzymes Immobilized Silicon Dioxide Carbon chemistry Yield (chemistry) biology.protein Cattle Graphite alpha-Amylases Isomerization Nuclear chemistry |
| Zdroj: | Journal of photochemistry and photobiology. B, Biology. 202 |
| ISSN: | 1873-2682 |
| Popis: | The photofunctionalization of three different carbons with two proteins was studied at room temperature. Water solutions of bovine serum albumin, BSA, and α-amylase, AA, were photolyzed at 21 °C in the presence of graphite microparticles (6.20 μm), MPG, graphene oxide, MPGO, and graphene oxide modified with SO2, mMPGO. The insertion of BSA on carbon matrixes occurred with a deoxygenation reaction, most likely due to a dehydration step of a water molecule. XPS, TOC and TGA, showed that the BSA photo-insertion on MPG was highly efficient with 34.9% of the weight of MPG after photolysis, with an initial concentration of 1 g∙L−1 of BSA. A high yield of AA photoinsertion on the carbons was also obtained. The calculated weight of AA inserted on MPG and MPGO after photolysis was 22.30% and 18.08%, respectively, with respect to the initial weight of carbon, when the initial concentration of AA was 60 mg∙L−1. AA immobilized on MPG was active while the enzyme on MPGO showed a smaller activity, within the experimental error. Although a certain extent of denaturalization of both proteins was observed during photolysis, the molecular weight and composition changed very little during the photolysis, which would produce mainly conformational changes and isomerization reactions. |
| Databáze: | OpenAIRE |
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