Development and validation of a spectroscopic method for the simultaneous analysis of miconazole nitrate and hydrocortisone acetate in pharmaceutical dosage form
Autor: | Muhammad Hafeez ur Rehman, Muhammad Arshad, Muhammad Fawad Rasool, Muhammad Ahsan, Muhammad Irfan, Nasir Abbas, Amjad Hussain |
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Rok vydání: | 2017 |
Předmět: |
Detection limit
Chromatography Serial dilution 010405 organic chemistry Chemistry 010401 analytical chemistry Pharmaceutical Science Simultaneous analysis Miconazole nitrate Hdrocortisone acetate UV-visible spectroscopy Method validation 01 natural sciences Dosage form 0104 chemical sciences Absorbance Ultraviolet visible spectroscopy Miconazole Nitrate medicine Pharmacology (medical) Miconazole Quantitative analysis (chemistry) medicine.drug |
Zdroj: | Tropical Journal of Pharmaceutical Research; Vol 16, No 2 (2017); 413-420 |
ISSN: | 1596-9827 1596-5996 |
Popis: | Purpose : To develop a new analytical method for the quantitative analysis of miconazole nitrate (MCN) and hydrocortisone (HCA) in pharmaceutical creams. Methods : The study was accomplished in three steps. In the first stage, a spectroscopic method was developed for the simultaneous analysis of miconazole (MCN) and hydrocortisone (HCA). For this purpose, serial dilutions of both drugs were prepared using ethanol: water (50:50) as the solvent system. All solutions were analyzed for absorbance spectrophotometrically at 205 and 249 nm for MCN and HCA, respectively. The method was validated for linearity, range, accuracy, precision and robustness. A commercial formulation was also assayed by the proposed method. Results : The absorbance data were linear over a concentration range of 1 to 12 μg/mL for MCN and 1 to 40 μg/mL for HCA, with correlation coefficient of 0.9995 and 0.9984, respectively. Limit of detection (LOD) and of quantification (LOQ) of MCN were 0.49 and 1.49 μg/mL, respectively, and for HCA, 1.50 and 4.57 μg/mL, respectively. The assay results for the commercial formulation were within the limits specified in British Pharmacopoeia. Conclusion : Simultaneous quantification of miconazole (MCN) and hydrocortisone (HCA) by UV spectroscopy provides a simple, reliable and robust method for the characterization of a mixture of the drugs in a dosage form. Keywords : Simultaneous analysis, Miconazole nitrate, Hdrocortisone acetate, UV-visible spectroscopy, Method validation |
Databáze: | OpenAIRE |
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